Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HDAC1 antibody [EPR5517(2)] - BSA and Azide free (ab248968)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5517(2)] to HDAC1 - BSA and Azide free
  • Suitable for: IHC-P, IP, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-HDAC1 antibody [EPR5517(2)] - BSA and Azide free
    See all HDAC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5517(2)] to HDAC1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: Jurkat whole cell lysate.

  • General notes

    Ab248968 is the carrier-free version of ab150399. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248968 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HDAC1 antibody [EPR5517(2)] - BSA and Azide free (ab248968)
    Western blot - Anti-HDAC1 antibody [EPR5517(2)] - BSA and Azide free (ab248968)

    This data was developed using ab150399, the same antibody clone in a different buffer formulation.

    Lane 1 Wild-type HAP1 cell lysate (20 µg)

    Lane 2 HDAC1 knockout HAP1 cell lysate (20 µg) 

    Lane 3 HeLa cell lysate (20 µg)

    Lane 4 Human breast carcinoma lysate (20 µg) 

    Lanes 1 - 4 Merged signal (red and green). Green - ab150399 observed at 65 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab150399 was shown to recognize HDAC1 when HDAC1 knockout samples were used, along with additional cross-reactive bands. Wild-type and HDAC1 knockout samples were subjected to SDS-PAGE. ab150399 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Immunoprecipitation - Anti-HDAC1 antibody [EPR5517(2)] - BSA and Azide free (ab248968)
    Immunoprecipitation - Anti-HDAC1 antibody [EPR5517(2)] - BSA and Azide free (ab248968)

    This data was developed using ab150399, the same antibody clone in a different buffer formulation.
    Purified ab150399 at 1/30 dilution (2µg) immunoprecipitating HDAC1 in Jurkat whole cell lysate.
    Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
    Lane 2 (+): ab150399 + Jurkat whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab150399 in Jurkat whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 62 kDa
    Faint band above 62kDa could be Sumoylated HDAC1. (PMID: 28186506)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] - BSA and Azide free (ab248968)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] - BSA and Azide free (ab248968)

    This data was developed using ab150399, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling HDAC1 with ab150399 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-HDAC1 antibody [EPR5517(2)] - BSA and Azide free (ab248968)
    Western blot - Anti-HDAC1 antibody [EPR5517(2)] - BSA and Azide free (ab248968)
    All lanes : Anti-HDAC1 antibody [EPR5517(2)] (ab150399) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 55 kDa



    This data was developed using ab150399, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-HDAC1 antibody [EPR5517(2)] - BSA and Azide free (ab248968)
    Western blot - Anti-HDAC1 antibody [EPR5517(2)] - BSA and Azide free (ab248968)

    This data was developed using ab150399, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: HDAC1 knockout HAP1 cell lysate (20 µg)

    Lane 3: HeLa cell lysate (20 µg)

    Lane 4: Human breast carcinoma lysate (20 µg) or K562 lysate (20 µg) 

    Lanes 1 - 4: Merged signal (red and green). Green - ab150399 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.

    This western blot image is a comparison between ab150399 and a competitor's top cited rabbit polyclonal antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] - BSA and Azide free (ab248968)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] - BSA and Azide free (ab248968)
    This data was developed using ab150399, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab150399 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] - BSA and Azide free (ab248968)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] - BSA and Azide free (ab248968)
    This data was developed using ab150399, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human stomach tissue using ab150399 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] - BSA and Azide free (ab248968)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] - BSA and Azide free (ab248968)
    This data was developed using ab150399, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab150399 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] - BSA and Azide free (ab248968)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] - BSA and Azide free (ab248968)
    This data was developed using ab150399, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab150399 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] - BSA and Azide free (ab248968)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR5517(2)] - BSA and Azide free (ab248968)
    This data was developed using ab150399, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human Thyroid gland carcinoma tissue using ab150399 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-HDAC1 antibody [EPR5517(2)] - BSA and Azide free (ab248968)
    Anti-HDAC1 antibody [EPR5517(2)] - BSA and Azide free (ab248968)

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