Anti-SIRT3 antibody [EPR23514-36] (ab246522)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23514-36] to SIRT3
- Suitable for: WB, IP
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-SIRT3 antibody [EPR23514-36]
See all SIRT3 primary antibodies -
Description
Rabbit monoclonal [EPR23514-36] to SIRT3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseRatWB MouseRat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse heart, liver and kidney tissue lysate; Rat liver and kidney tissue lysate. IP: Mouse and rat liver lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23514-36 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-SIRT3 antibody [EPR23514-36] (ab246522) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate 20 µg
Lane 2 : Mouse kidney tissue lysate 20 µg
Lane 3 : Mouse spleen tissue lysate 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 43 kDaBlocking/diluting buffer and concentration: 5% NFDM/TBST.
Negative control: Spleen (PMID:25566066).
Exposure time: 15 secs.
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SIRT3 was immunoprecipitated from 0.35 mg mouse liver lysate with ab246522 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246522 at a 1/1000 dilution (0.45 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse liver lysate 10μg
Lane 2: ab246522 IP in mouse liver lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246522 in mouse liver lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 secs.
The target band size is very close to IgG light chain.
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All lanes : Anti-SIRT3 antibody [EPR23514-36] (ab246522) at 1/1000 dilution
Lane 1 : Rat liver tissue lysate 20 µg
Lane 2 : Rat kidney tissue lysate 20 µg
Lane 3 : Rat spleen tissue lysate 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 43 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?Blocking/diluting buffer and concentration: 5% NFDM/TBST
Negative control: Spleen (PMID:25566066).
Exposure time: 15 secs.
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SIRT3 was immunoprecipitated from 0.35 mg rat liver lysate with ab246522 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246522 at a 1/1000 dilution (0.45 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat liver lysate 10μg
Lane 2: ab246522 IP in rat liver lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246522 in rat liver lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 secs.
The target band size is very close to IgG light chain.
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Anti-SIRT3 antibody [EPR23514-36] (ab246522) at 1/1000 dilution + Mouse liver tissue lysate 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 43 kDaBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 secs.
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