Anti-HDAC5 (phospho S498) antibody (ab47283)
Key features and details
- Rabbit polyclonal to HDAC5 (phospho S498)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HDAC5 (phospho S498) antibody
See all HDAC5 primary antibodies -
Description
Rabbit polyclonal to HDAC5 (phospho S498) -
Host species
Rabbit -
Specificity
This antibody is specific for HDAC5, only when phosphorylated at Serine 498. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse
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Immunogen
Synthetic peptide corresponding to Human HDAC5 (phospho S498). Immunogen range: 464-513
Database link: Q9UQL6 -
Positive control
- NIH/3T3 cell extracts; Breast carcinoma tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HDAC5 (phospho S498) antibody (ab47283)All lanes : Anti-HDAC5 (phospho S498) antibody (ab47283) at 1/500 dilution
Lane 1 : Extracts from NIH/ 3T3 cells
Lane 2 : Extracts from NIH/ 3T3 cells; ab47283 preincubated with immunising peptide (blocking)
Predicted band size: 122 kDa
Observed band size: 118 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC5 (phospho S498) antibody (ab47283)ab47283 (1/100) staining HDAC5 (phospho S498) in paraffin embedded human breast carcinoma tissue. For the right hand panel, ab47283 was pre-incubated with the immunizing peptide (blocking).
