Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸ 10 µl 107 212 ₸

Order now and get it on Wednesday March 17, 2021

Anti-HDAC5 (phospho S498) antibody [EPR22996-105] (ab240644)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22996-105] to HDAC5 (phospho S498)
Suitable for: WB, Dot blot
Reacts with: Mouse, Human

Product name

Anti-HDAC5 (phospho S498) antibody [EPR22996-105]
See all HDAC5 primary antibodies
Description

Rabbit monoclonal [EPR22996-105] to HDAC5 (phospho S498)
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, NIH/3T3 and C2C12, whole lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22996-105
Isotype

IgG
Research areas

Western blot - Anti-HDAC5 antibody [EPR22996-105] (ab240644)

All lanes : Anti-HDAC5 (phospho S498) antibody [EPR22996-105] (ab240644) at 1/1000 dilution

Lane 1 : C2C12 (mouse myoblasts myoblast), whole cell lysate (untreated membrane)
Lane 2 : C2C12, whole cell lysate (phosphatase treated membrane)

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 121 kDa
Observed band size: 140 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Western blot - Anti-HDAC5 antibody [EPR22996-105] (ab240644)

All lanes : Anti-HDAC5 (phospho S498) antibody [EPR22996-105] (ab240644) at 1/1000 dilution

Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate (untreated membrane)
Lane 2 : NIH/3T3 whole cell lysate (phosphatase treated membrane)

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 121 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Western blot - Anti-HDAC5 antibody [EPR22996-105] (ab240644)

All lanes : Anti-HDAC5 (phospho S498) antibody [EPR22996-105] (ab240644) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate (untreated membrane)
Lane 2 : HeLa whole cell lysate (phosphatase treated membrane)

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 121 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Dot Blot - Anti-HDAC5 (phospho S498) antibody [EPR22996-105] (ab240644)

Dot blot analysis of HDAC5 (phospho S498) labeled with ab240644 at 1/1000 dilution.

Lane 1: HDAC5 (phospho S498) peptide (aa495-504).

Lane 2: HDAC5 non-phospho peptide (aa493-504).

Lane 3: HDAC4 (phospho S632) peptide (aa627-638).

Lane 4: HDAC7 (phospho S486) peptide (aa481-492).

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution was used as secondary antibody.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Anti-HDAC5 (phospho S498) antibody [EPR22996-105] (ab240644)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com