Anti-HDAC5 antibody (ab1439)
Key features and details
- Rabbit polyclonal to HDAC5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HDAC5 antibody
See all HDAC5 primary antibodies -
Description
Rabbit polyclonal to HDAC5 -
Host species
Rabbit -
Specificity
The antibody detects ~124 kDa histone deacetylase 5. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 6, 7, and 8. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human HDAC5. The exact sequence is proprietary. Synthetic peptide surrounding amino acid 575
Database link: Q9UQL6 -
General notes
Some tissues may not express the HDAC-4 and 5 protein or it is expressed at extremely low amount. HDAC-1,2,3,6,7 are usually much more abundant (could be ~100X more) than HDAC-4 & 5. We would strongly suggest using positive control (HeLa lysate).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 30% Glycerol (glycerin, glycerine), 0.5% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HDAC5 antibody (ab1439)Anti-HDAC5 antibody (ab1439) at 1/1000 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 100 µg
Secondary
Goat Anti-Rabbit HRP at 1/10000 dilution
Predicted band size: 121.9 kDa
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Western blot - Anti-HDAC5 antibody (ab1439)All lanes : Anti-HDAC5 antibody (ab1439)
Lane 1 : Cell lysate prepared from HeLa cells
Lane 2 : Cell lysate prepared from HepG2 cells
Predicted band size: 121.9 kDa
Observed band size: 124 kDa why is the actual band size different from the predicted?
Western blot analysis of HDAC5 in HeLa (Lane1) and HepG2 (Lane2) cell lysates. A band of approximately 124 KDa can be detected.
