Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23847-170] to HDAC1 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free
    See all HDAC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23847-170] to HDAC1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ChIP
    Mouse
    Flow Cyt
    Mouse
    ICC
    Mouse
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human heart and kidney tissue lysates; Jurkat, K-562, HeLa, C6, RAW264.7, PC-12 and NIH/3T3 whole cell lysates; His-tagged human HDAC1 recombinant protein. IHC-P: Human tonsil and liver tissue; Mouse liver tissue; Rat liver tissue. ICC: HeLa and NIH/3T3 cells. Flow Cyt: HeLa cells and NIH/3T3 cells. IP: HeLa and NIH/3T3 whole cell lysates. ChIP: Chromatin prepared from K-562 cells.

  • General notes

    ab280205 is the carrier-free version of ab280198. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280205 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • ChIP - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)
    ChIP - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)

    This data was developed using ab280198, the same antibody clone in a different buffer formulation.

    Chromatin was prepared from K-562 cells according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab280198 (red), or 5 µg of rabbit normal IgG ab172730 (gray) and 25 µl of Protein A/G Dynabeads. The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol

  • Immunoprecipitation - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)
    Immunoprecipitation - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)

    This data was developed using ab280198, the same antibody clone in a different buffer formulation.

    HDAC1 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab280198 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280198 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug

    Lane 2: ab280198 IP in NIH/3T3 whole cell lysate 10 ug

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab280198 in NIH/3T3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second

     

  • Immunocytochemistry - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)
    Immunocytochemistry - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)

    This data was developed using ab280198, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling HDAC1 with ab280198 at 1/5000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in HeLa cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)

    This data was developed using ab280198, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling HDAC1 with ab280198 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human tonsil (PMID:23109994). The section was incubated with ab280198 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Flow Cytometry - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)
    Flow Cytometry - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)

    This data was developed using ab280198, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling HDAC1 with ab280198 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)
    Western blot - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)
    All lanes : Anti-HDAC1 antibody [EPR23847-170] (ab280198) at 1/1000 dilution

    Lane 1 : Human heart tissue lysate
    Lane 2 : Human kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 55 kDa
    Observed band size: 62 kDa why is the actual band size different from the predicted?



    This data was developed using ab280198, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID:24551070).

    Exposure time: 15 seconds

     

  • Western blot - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)
    Western blot - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)
    All lanes : Anti-HDAC1 antibody [EPR23847-170] (ab280198) at 1/5000 dilution

    Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
    Lane 2 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 55 kDa
    Observed band size: 62 kDa why is the actual band size different from the predicted?



    This data was developed using ab280198, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID: 24551070).

    Exposure time: 37 seconds

  • Immunocytochemistry - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)
    Immunocytochemistry - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)

    This data was developed using ab280198, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling HDAC1 with ab280198 at 1/5000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in NIH/3T3 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)
    Western blot - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)
    All lanes : Anti-HDAC1 antibody [EPR23847-170] (ab280198) at 1/1000 dilution

    Lane 1 : His-tagged human HDAC1 recombinant protein (aa1-482)
    Lane 2 : His-tagged human HDAC2 recombinant protein (aa1-488)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 55 kDa
    Observed band size: 66 kDa why is the actual band size different from the predicted?



    This data was developed using ab280198, the same antibody clone in a different buffer formulation. 

    This antibody has no cross-reaction with human HDAC2.

    These rec proteins were made in house. These two recombinant proteins were expressed from E.coli expression systems.

    Exposure time: 10 seconds

     

  • Flow Cytometry - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)
    Flow Cytometry - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)

    This data was developed using ab280198, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling HDAC1 with ab280198 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)

    This data was developed using ab280198, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling HDAC1 with ab280198 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human liver (PMID:18264140). The section was incubated with ab280198 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunoprecipitation - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)
    Immunoprecipitation - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)

    This data was developed using ab280198, the same antibody clone in a different buffer formulation.

    HDAC1 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab280198 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280198 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug

    Lane 2: ab280198 IP in HeLa whole cell lysate 10 ug

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab280198 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second

     

  • Western blot - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)
    Western blot - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)
    Lane 1 : Anti-HDAC1 antibody [EPR23847-170] (ab280198) at 1/1000 dilution
    Lanes 2-4 : Anti-HDAC1 antibody [EPR23847-170] (ab280198) at 1/5000 dilution

    Lane 1 : C6 (rat glial tumor glial cell) whole cell lysate
    Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 3 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 4 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 55 kDa
    Observed band size: 62 kDa why is the actual band size different from the predicted?



    This data was developed using ab280198, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID:24551070).

    Exposure time: 37 seconds

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)

    This data was developed using ab280198, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling HDAC1 with ab280198 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse liver. The section was incubated with ab280198 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR23847-170] - BSA and Azide free (ab280205)

    This data was developed using ab280198, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling HDAC1 with ab280198 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat liver. The section was incubated with ab280198 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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