Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TEM8/ATR antibody [EPNCI-R173-37] - BSA and Azide free (ab246321)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPNCI-R173-37] to TEM8/ATR - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-TEM8/ATR antibody [EPNCI-R173-37] - BSA and Azide free
    See all TEM8/ATR primary antibodies

  • Description

    Rabbit monoclonal [EPNCI-R173-37] to TEM8/ATR - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Fusion protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mammary fat pad of TEM8/ATR wildtype (WT) athymic nude mice transplanted with HT29 human colon tumor. ICC/IF: SW480 and LoVo cells.

  • General notes

    ab246321 is the carrier-free version of ab241067 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab246321 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as TEM8

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-TEM8/ATR antibody [EPNCI-R173-37] - BSA and Azide free (ab246321)
    Immunocytochemistry/ Immunofluorescence - Anti-TEM8/ATR antibody [EPNCI-R173-37] - BSA and Azide free (ab246321)

    Immunofluorescent analysis of 100% Methanol fixed LoVo (human colorectal adenocarcinoma epithelial cell) cells labeling TEM8/ATR with ab241067 at 1/100 dilution (Green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. Confocal image showing cytoplasmic and plasma membrane staining in LoVo cells.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    PFA fixation is not recommended due to weak staining on LoVo cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241067).

  • Immunocytochemistry/ Immunofluorescence - Anti-TEM8/ATR antibody [EPNCI-R173-37] - BSA and Azide free (ab246321)
    Immunocytochemistry/ Immunofluorescence - Anti-TEM8/ATR antibody [EPNCI-R173-37] - BSA and Azide free (ab246321)

    Immunofluorescent analysis of 100% Methanol fixed SW480 (human colorectal adenocarcinoma epithelial cell) cells labeling TEM8/ATR with ab241067 at 1/100 dilution (Green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. Confocal image showing cytoplasmic and plasma membrane staining in SW480 cells.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    PFA fixation is not recommended due to negative staining on SW480 cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241067).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEM8/ATR antibody [EPNCI-R173-37] - BSA and Azide free (ab246321)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEM8/ATR antibody [EPNCI-R173-37] - BSA and Azide free (ab246321)

    Immunohistochemical analysis of 10% neutral-buffered formalin Mammary fat pad of TEM8/ATR wildtype (WT) or knockout (KO) athymic nude mice transplanted with HT29 human colon tumor tissue labeling TEM8/ATR with ab241067 at 1/60 dilution, followed by a Biotin anti-rabbit IgG secondsry antibody. Positive staining in stromal cells of transplanted HT29 human colon tumor growing in TEM8/ATR wildtype (WT) athymic nude mice is observed.

    Antigen retrieval: Retrieved with proteinase K.

    This image was kindly provided by our collaborator Dr. Brad St. Croix, National Cancer Institute (NCI), NIH, and published in J Clin Invest. 128: 2927–2943 (PMID:29863500).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241067).

  • Anti-TEM8/ATR antibody [EPNCI-R173-37] - BSA and Azide free (ab246321)
    Anti-TEM8/ATR antibody [EPNCI-R173-37] - BSA and Azide free (ab246321)

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