Anti-TEM8/ATR antibody [EPNCI-R173-37] (ab241067)
![Anti-TEM8/ATR antibody [EPNCI-R173-37] (ab241067)](https://www.abcam.com/ps/products/241/ab241067/Images/ab241067-3-241067ICC2.jpg "Anti-TEM8/ATR antibody [EPNCI-R173-37] (ab241067)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCI-R173-37] to TEM8/ATR
- Suitable for: IHC-P, ICC/IF
- Reacts with: Mouse, Human
Overview
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Product name
Anti-TEM8/ATR antibody [EPNCI-R173-37]
See all TEM8/ATR primary antibodies -
Description
Rabbit monoclonal [EPNCI-R173-37] to TEM8/ATR -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
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Immunogen
Fusion protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mammary fat pad of TEM8/ATR wildtype (WT) athymic nude mice transplanted with HT29 human colon tumor. ICC/IF: SW480 and LoVo cells.
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General notes
This product was previously labelled as TEM8
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPNCI-R173-37 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-TEM8/ATR antibody [EPNCI-R173-37] (ab241067)
Immunofluorescent analysis of 100% Methanol fixed LoVo (human colorectal adenocarcinoma epithelial cell) cells labeling TEM8/ATR with ab241067 at 1/100 dilution (Green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. Confocal image showing cytoplasmic and plasma membrane staining in LoVo cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
PFA fixation is not recommended due to weak staining on LoVo cells.
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![Immunocytochemistry/ Immunofluorescence - Anti-TEM8/ATR antibody [EPNCI-R173-37] (ab241067)](https://www.abcam.com/ps/products/241/ab241067/Images/ab241067-2-241067ICC1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TEM8/ATR antibody [EPNCI-R173-37] (ab241067)Immunofluorescent analysis of 100% Methanol fixed SW480 (human colorectal adenocarcinoma epithelial cell) cells labeling TEM8/ATR with ab241067 at 1/100 dilution (Green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. Confocal image showing cytoplasmic and plasma membrane staining in SW480 cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
PFA fixation is not recommended due to negative staining on SW480 cells.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEM8/ATR antibody [EPNCI-R173-37] (ab241067)](https://www.abcam.com/ps/products/241/ab241067/Images/ab241067-1-241067IHC1a.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEM8/ATR antibody [EPNCI-R173-37] (ab241067)Immunohistochemical analysis of 10% neutral-buffered formalin Mammary fat pad of TEM8/ATR wildtype (WT) or knockout (KO) athymic nude mice transplanted with HT29 human colon tumor tissue labeling TEM8/ATR with ab241067 at 1/60 dilution, followed by a Biotin anti-rabbit IgG secondsry antibody. Positive staining in stromal cells of transplanted HT29 human colon tumor growing in TEM8/ATR wildtype (WT) athymic nude mice is observed.
Antigen retrieval: Retrieved with proteinase K.
This image was kindly provided by our collaborator Dr. Brad St. Croix, National Cancer Institute (NCI), NIH, and published in J Clin Invest. 128: 2927–2943 (PMID:29863500).
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![Anti-TEM8/ATR antibody [EPNCI-R173-37] (ab241067)](https://www.abcam.com/ps/products/241/ab241067/Images/ab241067-5-benefits-of-recombinant-antibodies.png)
Anti-TEM8/ATR antibody [EPNCI-R173-37] (ab241067)
