Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3203(N)(B)] to G-CSF - BSA and Azide free
  • Suitable for: IP, ICC/IF, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free
    See all G-CSF primary antibodies

  • Description

    Rabbit monoclonal [EPR3203(N)(B)] to G-CSF - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, ICC/IF, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A549, K-562, HepG2, HeLa, MCF7, MOLT-4, PC-3, KM3, NCI-H460 and HT-1376 cell lysates; Mouse and rat brain lysates. ICC/IF: BxPC-3 and HT-1376 cells. IP: G-CSF IP in K562 cell lysate. Flow Cyt: K562 cells.

  • General notes

    Ab236157 is the carrier-free version of ab181053. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab236157 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)
    Western blot - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)
    All lanes : Anti-G-CSF antibody [EPR3203(N)(B)] (ab181053) at 1/1000 dilution

    Lane 1 : A549 (Human lung carcinoma epithelial cell) cell lysate
    Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) cell lysate
    Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) cell lysate
    Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma epithelial cell) cell lysate
    Lane 6 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cell lysate
    Lane 7 : PC-3 (Human prostate adenocarcinoma epithelial cell) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 22 kDa
    Observed band size: 25 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

     

     Exposure time: 4 s

  • Flow Cytometry - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)
    Flow Cytometry - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)

    Flow Cytometry analysis of K562 (human chronic myelogenous leukemia) cells labeling G-CSF with purified ab181053 at 1/200 dilution (10µg/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181053).

  • Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)
    Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)

    Immunocytochemistry/ Immunofluorescence analysis of BxPC-3 (Human pancreas adenocarcinoma epithelial cell) cells labeling G-CSF with Purified ab181053 at 1:500 dilution (4.0μg/ml). Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear was used as a counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181053).

  • Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)
    Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)

    ab181053 (purified) at 1:100 dilution (2µg) immunoprecipitating G-CSF in K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate.
    Lane 1 (input): K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10µg
    Lane 2 (+): ab181053 and K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181053 in K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate.
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181053).

  • Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)
    Immunocytochemistry/ Immunofluorescence - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)

    Immunofluorescent analysis of HT-1376 cells (paraformaldehyde-fixed, 4%) labeling G-CSF with unpurified ab181053 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181053).

  • Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)
    Immunoprecipitation - Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)

    Western blot analysis of immunoprecipitation pellet from K562 cell lysate (lane 1) or a Negative control (lane 2)  immunoprecipitated using unpurified ab181053 at 1/20 dilution.

    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181053).

  • Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)
    Anti-G-CSF antibody [EPR3203(N)(B)] - BSA and Azide free (ab236157)

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