Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Signal Transduction Signaling Pathway Nuclear Signaling Nuclear Hormone Receptors Co-activators/co-repressors

Anti-BAF60C antibody [EPR5538(2)] - BSA and Azide free (ab249631)

Anti-BAF60C antibody [EPR5538(2)] - BSA and Azide free (ab249631)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5538(2)] to BAF60C - BSA and Azide free
  • Suitable for: IHC-P, IP, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Product image
Anti-PGC1 alpha antibody (ab54481)
Product image
FOXO1 Transcription Factor Assay Kit (Colorimetric) (ab207204)
Product image
Anti-FOXO1A (phospho S319) antibody (ab47326)
Product image
Recombinant Human PNRC2 protein (denatured) (ab167852)

Overview

  • Product name

    Anti-BAF60C antibody [EPR5538(2)] - BSA and Azide free
    See all BAF60C primary antibodies
  • Description

    Rabbit monoclonal [EPR5538(2)] to BAF60C - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, ICC/IF, WBmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab249631 is the carrier-free version of ab171075.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5538(2)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Swi / Snf
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • Other
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism

Images

  • Western blot - Anti-BAF60C antibody [EPR5538(2)] - BSA and Azide free (ab249631)
    Western blot - Anti-BAF60C antibody [EPR5538(2)] - BSA and Azide free (ab249631)
    All lanes : Anti-BAF60C antibody [EPR5538(2)] (ab171075) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : JAR cell lysate
    Lane 3 : C6 cell lysate
    Lane 4 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 55 kDa



    This data was developed using ab171075, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF60C antibody [EPR5538(2)] - BSA and Azide free (ab249631)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF60C antibody [EPR5538(2)] - BSA and Azide free (ab249631)
    This data was developed using ab171075, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human endometrial adenocarcinoma tissue labeling BAF60C with ab171075 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF60C antibody [EPR5538(2)] - BSA and Azide free (ab249631)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF60C antibody [EPR5538(2)] - BSA and Azide free (ab249631)
    This data was developed using ab171075, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human trachea tissue labeling BAF60C with ab171075 at 1/10 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-BAF60C antibody [EPR5538(2)] - BSA and Azide free (ab249631)
    Immunoprecipitation - Anti-BAF60C antibody [EPR5538(2)] - BSA and Azide free (ab249631)

    This data was developed using ab171075, the same antibody clone in a different buffer formulation.

    Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1XPBS (negative control) using ab171075 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.



    All lanes : Anti-BAF60C antibody [EPR5538(2)] (ab171075) at 1/10 dilution

    Lane 1 : Immunoprecipitation pellet from HeLa cell lysate
    Lane 2 : 1x PBS

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG
  • Anti-BAF60C antibody [EPR5538(2)] - BSA and Azide free (ab249631)
    Anti-BAF60C antibody [EPR5538(2)] - BSA and Azide free (ab249631)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

  •  
  • Product image

    Human GBA knockout HeLa cell lysate (ab256929)

  •  
  • Product image

    NADH Oxidase Activity Assay Kit (Fluorometric) (ab273345)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.