Anti-BAF60C antibody [EPR5538(2)] - BSA and Azide free (ab249631)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5538(2)] to BAF60C - BSA and Azide free
- Suitable for: IHC-P, IP, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-BAF60C antibody [EPR5538(2)] - BSA and Azide free
See all BAF60C primary antibodies -
Description
Rabbit monoclonal [EPR5538(2)] to BAF60C - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, ICC/IF, WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab249631 is the carrier-free version of ab171075.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR5538(2) -
Isotype
IgG -
Research areas
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
- Epigenetics and Nuclear Signaling
- Transcription
- Polymerase associated factors
- Pol II Transcription
- Other
Images
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All lanes : Anti-BAF60C antibody [EPR5538(2)] (ab171075) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : JAR cell lysate
Lane 3 : C6 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 55 kDaThis data was developed using ab171075, the same antibody clone in a different buffer formulation.
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This data was developed using ab171075, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human endometrial adenocarcinoma tissue labeling BAF60C with ab171075 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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This data was developed using ab171075, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human trachea tissue labeling BAF60C with ab171075 at 1/10 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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This data was developed using ab171075, the same antibody clone in a different buffer formulation.
Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1XPBS (negative control) using ab171075 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes : Anti-BAF60C antibody [EPR5538(2)] (ab171075) at 1/10 dilution
Lane 1 : Immunoprecipitation pellet from HeLa cell lysate
Lane 2 : 1x PBS
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
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