Human DKK4 ELISA Kit (ab243690)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 5.17 pg/ml
- Range: 21.88 pg/ml - 1400 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human DKK4 ELISA Kit
See all DKK4 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% supernatant 5 7.4% Inter-assay Sample n Mean SD CV% supernatant 3 2.9% -
Sample type
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
5.17 pg/ml -
Range
21.88 pg/ml - 1400 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 110 103% - 115% Serum 83 82% - 86% EDTA Plasma 92 87% - 98% Cit plasma 99 95% - 105% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human DKK4 ELISA Kit (ab243690) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DKK4 protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human DKK4 with 5.17 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human DKK4 Capture Antibody 1 x 600µl 10X Human DKK4 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human DKK4 Lyophilized recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. -
Tissue specificity
Expressed in cerebellum, T-cells, esophagus and lung. -
Sequence similarities
Belongs to the dickkopf family. -
Domain
The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6. -
Post-translational
modificationsAppears to be not glycosylated.
Can be proteolytically processed by a furin-like protease. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Dickkopf 4
- Dickkopf gene 4
- Dickkopf homolog 4
see all -
Database links
- Entrez Gene: 27121 Human
- GenBank: NP_055235.1 Human
- Omim: 605417 Human
- SwissProt: Q9UBT3 Human
- Unigene: 159311 Human
Images
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Other - Human DKK4 ELISA Kit (ab243690)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human DKK4 Standard Curve Comparison.Standard Curve comparison between human DKK4 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human DKK4 standard curve in Sample Diluent NS.The DKK4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native DKK4 in human SW480 cell culture supernatant samples.The concentrations of DKK4 were measured in duplicates, interpolated from the DKK4 standard curves and corrected for sample dilution. Undiluted samples are as follows: SW480 supernatant 100 %. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DKK4 concentration was determined to be 1,245 pg/mL in SW480 supernatant.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DKK4 concentration was determined to be 61.9 pg/mL with a range of ND – 97.5 pg/mL. ND = not detected, below the range of the assay.
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Serum from eight individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DKK4 concentration was determined to be 43.0 pg/mL with a range of ND – 113.2 pg/mL. ND = not detected, below the range of the assay.
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Sandwich ELISA - Human DKK4 ELISA Kit (ab243690)To learn more about the advantages of recombinant antibodies see here.
