Human DKK4 Antibody Pair - BSA and Azide free (ab256645)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human DKK4 Antibody Pair - BSA and Azide free
See all DKK4 kits -
Assay type
ELISA set -
Range
21.88 pg/ml - 1400 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human DKK4. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab243690), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human DKK4 Capture Antibody (unconjugated) 1 x 100µg Human DKK4 Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. -
Tissue specificity
Expressed in cerebellum, T-cells, esophagus and lung. -
Sequence similarities
Belongs to the dickkopf family. -
Domain
The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6. -
Post-translational
modificationsAppears to be not glycosylated.
Can be proteolytically processed by a furin-like protease. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Dickkopf 4
- Dickkopf gene 4
- Dickkopf homolog 4
see all -
Database links
- Entrez Gene: 27121 Human
- GenBank: NP_055235.1 Human
- Omim: 605417 Human
- SwissProt: Q9UBT3 Human
- Unigene: 159311 Human
Images
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Sandwich ELISA - Human DKK4 Antibody Pair - BSA and Azide free (ab256645)To learn more about the advantages of recombinant antibodies see here.
