Anti-AU1 tag antibody (ab3401)
Key features and details
- Rabbit polyclonal to AU1 tag
- Suitable for: ELISA, ICC, WB, IP
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-AU1 tag antibody -
Description
Rabbit polyclonal to AU1 tag -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, ICC, WB, IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AU1 tag antibody (ab3401)
The AU1 tag antibody (ab3401) was used in a Western blot to detect expression of mutant mammalian target of rapamycin (mTOR) proteins. U373 and A172 human glioma cell lines were transfected with constructs encoding either an AU1 tagged rapamycin resistant (RR) mTOR or AU1 tagged kinase dead (KD) mTOR. This detected the expression of a 290 kD protein corresponding to the AU1 tagged mutant mTOR proteins in both cell lines. (The mutant mTOR proteins appear beneath a band corresponding to the wild-type (WT) mTOR protein - this was not detected by ab3401 but a different antibody).
Picture from review by Amith Panner, submitted 29 July 2004.
The AU1 tag antibody (ab3401) was used in a Western blot to detect expression of mutant mammalian target of rapamycin (mTOR) proteins. U373 and A172 human glioma cell lines were transfected with constructs encoding either an AU1 tagged rapamycin resistant (RR) mTOR or AU1 tagged kinase dead (KD) mTOR. This detected