Vector contains no plant selection gene, kanamycin resistance gene for bacterial selection, the GusPlus™ reporter gene and the GRP signal peptide for in planta secretion.

The pCambia0305.2 vector offers:
High copy number in E.coli for high DNA yields
pVS1 replicon for high stability in Agrobacterium
Small size
Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest
Bacterial selection with kanamycin
the GRP-GUSPlus gene for secreted GUS activity

Reporter genes feature a hexa-Histidine tag at the C-terminus to enable simple purification on immobilized metal affinity chromatography resins.

Concentration of Antibiotics for selection.
In bacterial system:
Kanamycin resistance (aphIII/nptIII/3’5’’-aminoglycoside phosphotransferase typeIII from Enterococcus faecalis pJH1) in bacteria: use 50μg/mL in E.coli and A.tumefaciens.

In plant system: