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Mouse Apo B ELISA Kit (ab230932)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 196 pg/ml
  • Range: 1.56 ng/ml - 100 ng/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse Apo B ELISA Kit
    See all Apolipoprotein B kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 2.4%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 3.9%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    196 pg/ml

  • Range

    1.56 ng/ml - 100 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 83 75% - 95%
    Cell culture media 104 99% - 107%
    Hep Plasma 98 91% - 107%
    EDTA Plasma 88 79% - 98%
    Cit plasma 86 75% - 99%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Mouse Apo B ELISA Kit (ab230932) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Apo B protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Apo B with 196 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.


    ASSAY SPECIFICITY


    This kit recognizes both native and recombinant mouse ApoB protein in serum, plasma, and cell culture supernatant, cell and tissue extract samples only.



    SPECIES REACTIVITY


    This kit recognizes native and recombinant mouse ApoB protein. Other species reactivity was determined by measuring human serum samples, interpolating the protein concentrations from the standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in serum assayed at the same dilution. Reactivity was determined for Human

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse Apo B ELISA Kit (ab230932)
    Other - Mouse Apo B ELISA Kit (ab230932)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of ApoB standard curve in Sample Diluent NS.
    Example of ApoB standard curve in Sample Diluent NS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Standard curve
    Standard curve

    Example of ApoB standard curve in Sample Diluent NS. The mouse ApoB standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native mouse ApoB in serum and plasma samples.
    Interpolated concentrations of native mouse ApoB in serum and plasma samples.

    The concentrations of ApoB were measured in duplicate, interpolated from the mouse ApoB standard curve and corrected for sample dilution. Undiluted samples are as follows: serum 1:2000, plasma (citrate) 1:2000, plasma (EDTA) 1:2000, and plasma (heparin) 1:2000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ApoB concentration was determined to be 187.00 µg/mL in serum, 114.525 µg/mL in plasma (citrate) and 149.723 µg/mL in plasma (EDTA), and 152.208 µg/mL in plasma (Heparin).

  • Linearity of dilution.
    Linearity of dilution.

    Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

    Native mouse ApoB was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

    Recombinant ApoB was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.

  • Sandwich ELISA - Mouse Apo B ELISA Kit (ab230932)
    Sandwich ELISA - Mouse Apo B ELISA Kit (ab230932)
    To learn more about the advantages of recombinant antibodies see here.

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