Mouse Apo B ELISA Kit (ab230932)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 196 pg/ml
- Range: 1.56 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Apo B ELISA Kit
See all Apolipoprotein B kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 2.4% Inter-assay Sample n Mean SD CV% Serum 3 3.9% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
196 pg/ml -
Range
1.56 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 83 75% - 95% Cell culture media 104 99% - 107% Hep Plasma 98 91% - 107% EDTA Plasma 88 79% - 98% Cit plasma 86 75% - 99% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Apo B ELISA Kit (ab230932) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Apo B protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Apo B with 196 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY
This kit recognizes both native and recombinant mouse ApoB protein in serum, plasma, and cell culture supernatant, cell and tissue extract samples only.
SPECIES REACTIVITYThis kit recognizes native and recombinant mouse ApoB protein. Other species reactivity was determined by measuring human serum samples, interpolating the protein concentrations from the standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in serum assayed at the same dilution. Reactivity was determined for Human
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse ApoB Capture Antibody 1 x 600µl 10X Mouse ApoB Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Mouse ApoB Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. -
Involvement in disease
Hypobetalipoproteinemia, familial, 1
Familial ligand-defective apolipoprotein B-100
Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia. -
Sequence similarities
Contains 1 vitellogenin domain. -
Post-translational
modificationsPalmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle. -
Cellular localization
Cytoplasm. Secreted. - Information by UniProt
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Alternative names
- Apo B
- Apo B 100
- Apo B-100
see all -
Database links
- Entrez Gene: 238055 Mouse
- SwissProt: E9Q414 Mouse
- Unigene: 221239 Mouse
Images
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Other - Mouse Apo B ELISA Kit (ab230932)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of ApoB standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Standard curveExample of ApoB standard curve in Sample Diluent NS. The mouse ApoB standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native mouse ApoB in serum and plasma samples.The concentrations of ApoB were measured in duplicate, interpolated from the mouse ApoB standard curve and corrected for sample dilution. Undiluted samples are as follows: serum 1:2000, plasma (citrate) 1:2000, plasma (EDTA) 1:2000, and plasma (heparin) 1:2000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ApoB concentration was determined to be 187.00 µg/mL in serum, 114.525 µg/mL in plasma (citrate) and 149.723 µg/mL in plasma (EDTA), and 152.208 µg/mL in plasma (Heparin).
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Linearity of dilution.Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native mouse ApoB was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Recombinant ApoB was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.
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Sandwich ELISA - Mouse Apo B ELISA Kit (ab230932)To learn more about the advantages of recombinant antibodies see here.
