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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Methylation

Anti-RTF1 antibody (ab99362)

Anti-RTF1 antibody (ab99362)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to RTF1
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-RTF1 antibody
    See all RTF1 primary antibodies
  • Description

    Rabbit polyclonal to RTF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis, Medaka fish
  • Immunogen

    Immunogen corresponding to C terminal amino acids within Human RTF1 (EAW92492.1).

  • Positive control

    • HeLa whole cell lysate (ab150035).
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Elongation factors
    • Microbiology
    • Organism
    • Eukaryotic Microorganism
    • Yeast
    • Saccharomyces spp
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers

Images

  • Western blot - Anti-RTF1 antibody (ab99362)
    Western blot - Anti-RTF1 antibody (ab99362)
    All lanes : Anti-RTF1 antibody (ab99362) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 77 kDa


    Exposure time: 10 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody (ab99362)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody (ab99362)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma (left) and mouse teratoma (right) tissues labelling RTF1 with ab99362 at 1/1000 (1µg/ml). Detection: DAB.
  • Immunoprecipitation - Anti-RTF1 antibody (ab99362)
    Immunoprecipitation - Anti-RTF1 antibody (ab99362)
    Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab99362 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane

    Western Blot: RTF1 antibody (ab99362) at 1 µg/ml

    developed using the ECL technique

    Exposure time : 3 seconds

    Predicted band size : 77 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-RTF1 antibody (ab99362)

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    Anti-RTF1 antibody (ab70690)

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    Applications: ICC/IF, IHC-P, WB

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    Anti-RTF1 antibody (ab231291)

    Applications: WB

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    Anti-RTF1 antibody (ab84564)

    Applications: IHC-P

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