HRP Anti-ERG antibody [EPR3864] (ab195514)
![HRP Anti-ERG antibody [EPR3864] (ab195514)](https://www.abcam.com/ps/products/195/ab195514/Images/ab195514-357050-ID1317ab199514AP4928822DatasheetImage2019.jpg "HRP Anti-ERG antibody [EPR3864] (ab195514)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR3864] to ERG
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-ERG antibody [EPR3864]
See all ERG primary antibodies -
Description
HRP Rabbit monoclonal [EPR3864] to ERG -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Dissociation constant (KD)
KD = 8.90 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3864 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-ERG antibody [EPR3864] (ab195514)HRP Anti-ERG antibody [EPR3864] (ab195514) at 1/1000 dilution + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab195514 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-ERG antibody [EPR3864] (ab195514)](https://www.abcam.com/ps/products/195/ab195514/Images/ab195514-3-benefits-of-recombinant-antibodies.png)
HRP Anti-ERG antibody [EPR3864] (ab195514)
