Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RTF1 antibody [EPR13615] (ab184570)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13615] to RTF1
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-RTF1 antibody [EPR13615]
    See all RTF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13615] to RTF1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293, A431, HepG2 or HeLa lysate, Human papillary adenocarcinoma of thyroid or stomach tissue, HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-RTF1 antibody [EPR13615] (ab184570)
    Western blot - Anti-RTF1 antibody [EPR13615] (ab184570)
    Anti-RTF1 antibody [EPR13615] (ab184570) at 1/5000 dilution + HeLa lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 80 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RTF1 antibody [EPR13615] (ab184570)
    Immunocytochemistry/ Immunofluorescence - Anti-RTF1 antibody [EPR13615] (ab184570)

    Immunofluorescence analysis of HeLa cells (fixative 4% paraformaldehyde) labeling RTF1 with ab184570 at a 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor® 488) secondary used at a 1/200 diution (left image) and conterstained with Dapi (right image).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody [EPR13615] (ab184570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody [EPR13615] (ab184570)

    Immunohistochemical analysis of paraffin embedded Human stomach tissue labeling RTF1 with ab184570 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody [EPR13615] (ab184570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody [EPR13615] (ab184570)

    Immunohistochemical analysis of paraffin embedded Human papillary adenocarcinoma of thyroid tissue labeling RTF1  with ab184570 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-RTF1 antibody [EPR13615] (ab184570)
    Western blot - Anti-RTF1 antibody [EPR13615] (ab184570)
    All lanes : Anti-RTF1 antibody [EPR13615] (ab184570) at 1/1000 dilution

    Lane 1 : 293 lysate
    Lane 2 : A431 lysate
    Lane 3 : HepG2 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 80 kDa

  • Anti-RTF1 antibody [EPR13615] (ab184570)
    Anti-RTF1 antibody [EPR13615] (ab184570)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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