Anti-GABARAP antibody - N-terminal (ab227516)
Images
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Immunocytochemistry/ Immunofluorescence - Anti-GABARAP antibody - N-terminal (ab227516)
U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells stained for GABARAP (green) using ab227516 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Blue: Hoechst 33342 staining.
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Western blot - Anti-GABARAP antibody - N-terminal (ab227516)All lanes : Anti-GABARAP antibody - N-terminal (ab227516) at 1/3000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HEK-293T transfected with GABARAP shRNA, whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 14 kDa15% SDS-PAGE
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Western blot - Anti-GABARAP antibody - N-terminal (ab227516)All lanes : Anti-GABARAP antibody - N-terminal (ab227516) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 (human brain neuroblast cell line) whole cell extract
Lane 4 : SK-N-AS (human neuroblastoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 14 kDa15% SDS-PAGE
