Anti-GABARAP antibody (ab223569)
Key features and details
- Rabbit polyclonal to GABARAP
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GABARAP antibody
See all GABARAP primary antibodies -
Description
Rabbit polyclonal to GABARAP -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human GABARAP (C terminal).
Database link: O95166 -
Positive control
- WB: HEK-293T whole cell lysate; Mouse kidney lysate. ICC/IF: SK-N-BE cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-GABARAP antibody (ab223569)
4% formaldehyde-fixed SK-N-BE (human) cells stained for GABARAP (green) using ab223569 at 1/100 dilution in ICC/IF. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1/100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.
(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223569 (D) Merge.
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Western blot - Anti-GABARAP antibody (ab223569)Anti-GABARAP antibody (ab223569) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 14 kDa
Observed band size: 13.9,40 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutesBlocking buffer: 5% milk in TBST.
Color Development: TMB solution.
Primary and secondary antibodies were incubated for 1 hour at RT.
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Western blot - Anti-GABARAP antibody (ab223569)Anti-GABARAP antibody (ab223569) at 1/1000 dilution + Mouse kidney lysate at 20 µg
Secondary
Goat Anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 14 kDa
Observed band size: 13.9,40 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutesBlock: 5% milk + TBST
