Anti-PRKAR2 antibody (ab264220)
Key features and details
- Rabbit polyclonal to PRKAR2
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PRKAR2 antibody -
Description
Rabbit polyclonal to PRKAR2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PRKAR2 aa 50-100. The exact sequence is proprietary.
Database link: P13861 -
Positive control
- WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human colon carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-PRKAR2 antibody (ab264220)
PRKAR2 was immunoprecipitated from 1 mg HeLa whole cell lysate using ab264220 at 3 µg/mg lysate. Western blot was performed on the immunoprecipitate using ab264220 at 1 µg/ml.
Lane 1: ab264220 IP in HeLa whole cell lysate.
Lane 2: Control IgG. -
Western blot - Anti-PRKAR2 antibody (ab264220)All lanes : Anti-PRKAR2 antibody (ab264220) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 30 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2 antibody (ab264220)Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for PRKAR2 using ab264220 at 1/200 dilution in immunohistochemical analysis. Detection: DAB.