Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - BSA and Azide free (ab250860)
![Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - BSA and Azide free (ab250860)](https://www.abcam.com/ps/products/250/ab250860/Images/ab250860-396485-anti-annexin-2anxa2-antibody-epr130532b-c-terminal-western-blot-hela-wt-hepg2-hela-anxa2-knockout-shsy5y.jpg "Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - BSA and Azide free (ab250860)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13053(2)(B)] to Annexin-2/ANXA2 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - BSA and Azide free
See all Annexin-2/ANXA2 primary antibodies -
Description
Rabbit monoclonal [EPR13053(2)(B)] to Annexin-2/ANXA2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, HepG2 and SH-SY5Y cell lysates.
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General notes
ab250860 is the carrier-free version of ab185957 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab250860 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Annexin A2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13053(2)(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - BSA and Azide free (ab250860)All lanes : Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - C-terminal (ab185957) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ANXA2 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab185957).
Lanes 1 - 4: Merged signal (red and green). Green - ab185957 observed at 38 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab185957 was shown to react with Annexin-2/ANXA2 in wild-type HeLa cells in western blot. Loss of signal was observed when ANXA2 knockout sample was used. Wild-type and ANXA2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab185957 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - BSA and Azide free (ab250860)](https://www.abcam.com/ps/products/250/ab250860/Images/ab250860-2-benefits-of-recombinant-antibodies.png)
Anti-Annexin-2/ANXA2 antibody [EPR13053(2)(B)] - BSA and Azide free (ab250860)
