Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Annexin A1/ANXA1 antibody [2F1] (ab135256)

Key features and details

  • Mouse monoclonal [2F1] to Annexin A1/ANXA1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human, African green monkey, Recombinant fragment
  • Isotype: IgG1

Overview

  • Product name

    Anti-Annexin A1/ANXA1 antibody [2F1]
    See all Annexin A1/ANXA1 primary antibodies

  • Description

    Mouse monoclonal [2F1] to Annexin A1/ANXA1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human Annexin A1/ANXA1 aa 144-248.

  • Positive control

    • Human Annexin A1 recombinant protein; HeLa, A549, K562, NIH 3T3, C6 and COS7 cell lysates; Human cervical and ovarian cancer tissues.

Properties

Images

  • Western blot - Anti-Annexin A1/ANXA1 antibody [2F1] (ab135256)
    Western blot - Anti-Annexin A1/ANXA1 antibody [2F1] (ab135256)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: Annexin A1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)

     

    Lanes 1 - 4: Merged signal (red and green). Green - ab135256 observed at 38 kDa. Red - loading control, ab176560, observed at 50 kDa.

     

    ab135256 was shown to specifically react with Annexin A1 when Annexin A1 knockout samples were used. Wild-type and Annexin A1 knockout samples were subjected to SDS-PAGE. Ab135256 and ab176560 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [2F1] (ab135256)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [2F1] (ab135256)
    Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labelling Annexin A1 with ab135256 at 1/200 dilution followed by DAB staining.
  • Western blot - Anti-Annexin A1/ANXA1 antibody [2F1] (ab135256)
    Western blot - Anti-Annexin A1/ANXA1 antibody [2F1] (ab135256)
    Anti-Annexin A1/ANXA1 antibody [2F1] (ab135256) at 1/500 dilution + Human Annexin A1 recombinant protein.

    Predicted band size: 39 kDa



    Expected MW of recombinant fragment is 33.7 kDa.
  • Western blot - Anti-Annexin A1/ANXA1 antibody [2F1] (ab135256)
    Western blot - Anti-Annexin A1/ANXA1 antibody [2F1] (ab135256)
    All lanes : Anti-Annexin A1/ANXA1 antibody [2F1] (ab135256) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : NIH 3T3 cell lysate
    Lane 5 : C6 cell lysate
    Lane 6 : COS7 cell lysate

    Predicted band size: 39 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [2F1] (ab135256)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody [2F1] (ab135256)
    Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labelling Annexin A1 with ab135256 at 1/200 dilution followed by DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Annexin A1/ANXA1 antibody [2F1] (ab135256)

© 2021 Astana Biomed Group LLP. All rights reserved.