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Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [PO12/8] to Inhibin alpha - BSA and Azide free
  • Suitable for: IHC-P, ELISA, WB
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free
    See all Inhibin alpha primary antibodies

  • Description

    Mouse monoclonal [PO12/8] to Inhibin alpha - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, ELISA, WBmore details

  • Species reactivity

    Reacts with: Human, Recombinant fragment

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human testis and placenta tissue lysates. IHC-P: Human testis and ovary tissue. ELISA: Human Inhibin alpha.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab273486 is the carrier-free version of ab273454. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab273486 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)
    Western blot - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)
    All lanes : Anti-Inhibin alpha antibody [PO12/8] (ab273454) at 1/1000 dilution

    Lane 1 : Human testis tissue lysate
    Lane 2 : Human placenta tissue lysate
    Lane 3 : Human tonsil tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa



    This data was produced using the same clone but in a different formulation, ab273454.

    Negative control: Human tonsil.
    The molecular weight observed is consistent with what has been described in the literature (PMID:31658054).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 180 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)

    This data was produced using the same clone but in a different formulation, ab273454.

    Immunohistochemical analysis of paraffin-embedded human ovary tissue labeling Inhibin alpha with ab273454 at 1/1000 (0.937 µg/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplamic staining on the theca interna of the human ovary. The section was incubated with ab273454 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • ELISA - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)
    ELISA - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)

    This data was produced using the same clone but in a different formulation, ab273454.

    ab273454 used at 0-2000 ng/ml.

    Antigen (Human Inhibin alpha) used at 1000 ng/ml.

    Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at a 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)

    This data was produced using the same clone but in a different formulation, ab273454.

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Inhibin alpha with ab273454 at 1/1000 (0.937 µg/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplamic staining on human testis. The section was incubated with ab273454 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)
    Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (ab273486)

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