Anti-EBP1 antibody [EPR14568(B)] - BSA and Azide free (ab250244)
![Anti-EBP1 antibody [EPR14568(B)] - BSA and Azide free (ab250244)](https://www.abcam.com/ps/products/250/ab250244/Images/ab250244-2-anti-ebp1-antibody-epr14568-b-bsa-and-azide-free-flow-cytometry.jpg "Anti-EBP1 antibody [EPR14568(B)] - BSA and Azide free (ab250244)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14568(B)] to EBP1 - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-EBP1 antibody [EPR14568(B)] - BSA and Azide free
See all EBP1 primary antibodies -
Description
Rabbit monoclonal [EPR14568(B)] to EBP1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
Ab250244 is the carrier-free version of ab180603. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250244 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR14568(B) -
Isotype
IgG -
Research areas
Images
-
Flow Cytometry - Anti-EBP1 antibody [EPR14568(B)] - BSA and Azide free (ab250244)This data was developed using ab180603, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling EBP1 with ab180603 at 1/20 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) at 1/150 dilution.
-
![Western blot - Anti-EBP1 antibody [EPR14568(B)] - BSA and Azide free (ab250244)](https://www.abcam.com/ps/products/250/ab250244/Images/ab250244-398396-anti-ebp1-antibody-epr14568-b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-EBP1 antibody [EPR14568(B)] - BSA and Azide free (ab250244)All lanes : Anti-EBP1 antibody [EPR14568(B)] (ab180603) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 44 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?This data was developed using ab180603, the same antibody clone in a different buffer formulation.
-
![Anti-EBP1 antibody [EPR14568(B)] - BSA and Azide free (ab250244)](https://www.abcam.com/ps/products/250/ab250244/Images/ab250244-3-benefits-of-recombinant-antibodies.png)
Anti-EBP1 antibody [EPR14568(B)] - BSA and Azide free (ab250244)