Anti-GSDMD antibody [EPR20859-147] - BSA and Azide free (ab255985)
![Anti-GSDMD antibody [EPR20859-147] - BSA and Azide free (ab255985)](https://www.abcam.com/ps/products/255/ab255985/Images/ab255985-349783-anticleavedcterminalgsdmdantibodyepr20859147westernblotibmmmouse.jpg "Anti-GSDMD antibody [EPR20859-147] - BSA and Azide free (ab255985)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20859-147] to GSDMD - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse
Overview
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Product name
Anti-GSDMD antibody [EPR20859-147] - BSA and Azide free
See all GSDMD primary antibodies -
Description
Rabbit monoclonal [EPR20859-147] to GSDMD - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: iBMM (treated with 500ng/ml Bsak plus 500ng/ml anthrax protective antigen (PA) for 2h), concentrated cell supernatant .
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General notes
Ab255985 is the carrier-free version of ab255603. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab255985 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20859-147 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GSDMD antibody [EPR20859-147] - BSA and Azide free (ab255985)All lanes : Anti-cleaved C-terminal GSDMD antibody [EPR20859-147] (ab255603) at 1/1000 dilution
Lane 1 : iBMM (mouse immortalized bone marrow derived macrophages) treated with 500ng/ml Bsak plus 500ng/ml anthrax protective antigen (PA) for 2h. Cell lysate
Lane 2 : iBMM (mouse immortalized bone marrow derived macrophages) treated with 500ng/ml Bsak plus 500ng/ml anthrax protective antigen (PA) for 2h. Concentrated cell supernatant
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 53 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesLysates were kindly provided by Dr Feng Shao.
The molecular weight observed is consistent with literature (PMID: 26375003). C-terminal fragment is secreted and soluble (PMID: 27339137).
Blocking/Dilution buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255603).
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![Anti-GSDMD antibody [EPR20859-147] - BSA and Azide free (ab255985)](https://www.abcam.com/ps/products/255/ab255985/Images/ab255985-2-benefits-of-recombinant-antibodies.png)
Anti-GSDMD antibody [EPR20859-147] - BSA and Azide free (ab255985)
