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Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14204(B)] to E1 Ubiquitin Activating Enzyme 1/UBA1
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)]
    See all E1 Ubiquitin Activating Enzyme 1/UBA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14204(B)] to E1 Ubiquitin Activating Enzyme 1/UBA1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal brain, K562, HeLa and Jurkat lysates; Human kidney tissue; HeLa cells; K562 cells.

  • General notes

    This product was previously labelled as E1 Ubiquitin Activating Enzyme.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)
    Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)
    All lanes : Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225) at 1/10000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 118 kDa
    Observed band size: 118 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling E1 Ubiquitin Activating Enzyme with ab181225 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)
    Immunocytochemistry/ Immunofluorescence - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)

    Immunofluorescent analysis of acetone-fixed HeLa cells labeling E1 Ubiquitin Activating Enzyme with ab181225 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution (red). Counter stained with Dapi (blue).

  • Flow Cytometry - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)
    Flow Cytometry - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)

    Flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling E1 Ubiquitin Activating Enzyme with ab181225 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)
    Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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