Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 09, 2021

Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14204(B)] to E1 Ubiquitin Activating Enzyme 1/UBA1
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)]
See all E1 Ubiquitin Activating Enzyme 1/UBA1 primary antibodies
Description

Rabbit monoclonal [EPR14204(B)] to E1 Ubiquitin Activating Enzyme 1/UBA1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal brain, K562, HeLa and Jurkat lysates; Human kidney tissue; HeLa cells; K562 cells.
General notes
This product was previously labelled as E1 Ubiquitin Activating Enzyme.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR14204(B)
Isotype

IgG
Research areas

Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)

All lanes : Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225) at 1/10000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 118 kDa
Observed band size: 118 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling E1 Ubiquitin Activating Enzyme with ab181225 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)

Immunofluorescent analysis of acetone-fixed HeLa cells labeling E1 Ubiquitin Activating Enzyme with ab181225 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution (red). Counter stained with Dapi (blue).
Flow Cytometry - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)

Flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling E1 Ubiquitin Activating Enzyme with ab181225 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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