Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab264179)
Key features and details
- Rabbit polyclonal to E1 Ubiquitin Activating Enzyme 1/UBA1
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody
See all E1 Ubiquitin Activating Enzyme 1/UBA1 primary antibodies -
Description
Rabbit polyclonal to E1 Ubiquitin Activating Enzyme 1/UBA1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow
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Immunogen
Synthetic peptide aa 1-50. The exact sequence is proprietary.
Database link: P22314 -
Positive control
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates; IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab264179)All lanes : Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab264179) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Lane 5 : NIH/3T3 whole cell lysate at 50 µg
Secondary
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Exposure time: 30 seconds4-8% SDS-PAGE.
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Immunoprecipitation - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab264179)UBA1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (20% of IP loaded) using ab264179 at 3 µg/mg lysate. Western blot was performed on the immunprecipitate using ab264179 at 1 µg/ml.
Lane 1: ab264179 in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Detected with chemilimuniscence with exposure time of 30 seconds.
