Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (ab250441)
![Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (ab250441)](https://www.abcam.com/ps/products/250/ab250441/Images/ab250441-407851-anti-e1-ubiquitin-activating-enzyme-1-uba1-antibody-epr14204-b-bsa-and-azide-free-western-blot.jpg "Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (ab250441)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14204(B)] to E1 Ubiquitin Activating Enzyme 1/UBA1 - BSA and Azide free
- Suitable for: IHC-P, ICC, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free
See all E1 Ubiquitin Activating Enzyme 1/UBA1 primary antibodies -
Description
Rabbit monoclonal [EPR14204(B)] to E1 Ubiquitin Activating Enzyme 1/UBA1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250441 is the carrier-free version of ab181225 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab250441 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as E1 Ubiquitin Activating Enzyme
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR14204(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (ab250441)All lanes : Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225) at 1/10000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 118 kDa
Observed band size: 118 kDaThis data was developed using ab181225, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (ab250441)](https://www.abcam.com/ps/products/250/ab250441/Images/ab250441-3-anti-e1-ubiquitin-activating-enzyme-1-uba1-antibody-epr14204-b-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (ab250441)This data was developed using ab181225, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling E1 Ubiquitin Activating Enzyme with ab181225 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
![Immunocytochemistry - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (ab250441)](https://www.abcam.com/ps/products/250/ab250441/Images/ab250441-2-anti-e1-ubiquitin-activating-enzyme-1-uba1-antibody-epr14204-b-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (ab250441)This data was developed using ab181225, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of acetone-fixed HeLa cells labeling E1 Ubiquitin Activating Enzyme with ab181225 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution (red). Counter stained with Dapi (blue). -
![Flow Cytometry - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (ab250441)](https://www.abcam.com/ps/products/250/ab250441/Images/ab250441-1-anti-e1-ubiquitin-activating-enzyme-1-uba1-antibody-epr14204-b-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (ab250441)This data was developed using ab181225, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling E1 Ubiquitin Activating Enzyme with ab181225 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution. -
![Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (ab250441)](https://www.abcam.com/ps/products/250/ab250441/Images/ab250441-5-benefits-of-recombinant-antibodies.png)
Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (ab250441)
