Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

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Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] (ab180125)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14203(B)] to E1 Ubiquitin Activating Enzyme 1/UBA1
Suitable for: WB, Flow Cyt, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)]
See all E1 Ubiquitin Activating Enzyme 1/UBA1 primary antibodies
Description

Rabbit monoclonal [EPR14203(B)] to E1 Ubiquitin Activating Enzyme 1/UBA1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human E1 Ubiquitin Activating Enzyme 1/UBA1 aa 200-300. The exact sequence is proprietary.
Database link: P22314
Positive control
- Fetal brain, K562, HeLa and Jurkat cell lysates; Human ovarian carcinoma tissue; Jurkat and K562 cells.
General notes
This product was previously labelled as E1 Ubiquitin Activating Enzyme

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR14203(B)
Isotype

IgG
Research areas

Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] (ab180125)

All lanes : Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] (ab180125) at 1/5000 dilution

Lane 1 : Fetal brain lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 118 kDa
Observed band size: 117 kDa
why is the actual band size different from the predicted?
Flow Cytometry - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] (ab180125)

Flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling E1 Ubiquitin Activating Enzyme with ab180125 at 1/70 dilution (red) compared to a rabbit IgG control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/75 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] (ab180125)

Immunofluorescent analysis of acetone-fixed Jurkat cells labeling E1 Ubiquitin Activating Enzyme with ab180125 at 1/100 dilution, followed by Goat anti rabbit IgG (Dylight 488) at 1/250 dilution. Counter stained with DAPI (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] (ab180125)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling E1 Ubiquitin Activating Enzyme with ab180125 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] (ab180125)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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