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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin

Human DCAF12 knockout HeLa cell lysate (ab258839)

Price and availability

318 288 ₸

Availability

Order now and get it on Friday March 05, 2021

Human DCAF12 knockout HeLa cell lysate (ab258839)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

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Overview

  • Product name

    Human DCAF12 knockout HeLa cell lysate
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    HeLa
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 4 bp deletion in exon1 and Insertion of the selection cassette in exon1.
  • Passage number

  • Knockout validation

    Sanger Sequencing
  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab261364 - Human DCAF12 knockout HeLa cell lysate 1 x 100µg
    ab255929 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • RING Finger E3 Ligase
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female
  • STR Analysis

    Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10

Target

  • Function

    May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.
  • Tissue specificity

    Highly expressed in lung cancer tissues and some cancer cell lines. Restricted expression in normal testis.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the WD repeat DCAF12 family.
    Contains 4 WD repeats.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton > centrosome.
  • Target information above from: UniProt accession Q5T6F0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • cancer/testis antigen 102
    • Centrosome-related protein TCC52
    • CT102
    • DCA12_HUMAN
    • DCAF12
    • DDB1- and CUL4-associated factor 12
    • DKFZP434O125
    • KIAA1892
    • MGC1058
    • TCC52
    • Testis cancer centrosome-related protein
    • WD repeat domain 40A
    • WD repeat-containing protein 40A
    • WDR40A
    see all

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab261364 - Human DCAF12 knockout HeLa cell lysate 1 x 100µg
    ab255929 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • RING Finger E3 Ligase
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female
  • STR Analysis

    Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10

Images

  • Sanger Sequencing - Human DCAF12 knockout HeLa cell lysate (ab258839)
    Sanger Sequencing - Human DCAF12 knockout HeLa cell lysate (ab258839)

    Allele-1: 4 bp deletion in exon1

     

  • Sanger Sequencing - Human DCAF12 knockout HeLa cell lysate (ab258839)
    Sanger Sequencing - Human DCAF12 knockout HeLa cell lysate (ab258839)

    Allele-2: 1 bp deletion in exon1

     

  • Sanger Sequencing - Human DCAF12 knockout HeLa cell lysate (ab258839)
    Sanger Sequencing - Human DCAF12 knockout HeLa cell lysate (ab258839)

    Allele-3: Insertion of the selection cassette in exon1

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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