Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14203(B)] to E1 Ubiquitin Activating Enzyme 1/UBA1 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, ICC, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free
    See all E1 Ubiquitin Activating Enzyme 1/UBA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14203(B)] to E1 Ubiquitin Activating Enzyme 1/UBA1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, ICC, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250138 is the carrier-free version of ab180125.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)
    Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)
    All lanes : Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] (ab180125) at 1/5000 dilution

    Lane 1 : Fetal brain tissue lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 118 kDa
    Observed band size: 117 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab180125, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)
    Flow Cytometry - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)
    This data was developed using ab180125, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling E1 Ubiquitin Activating Enzyme with ab180125 at 1/70 dilution (red) compared to a rabbit IgG control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/75 dilution.
  • Immunocytochemistry - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)
    Immunocytochemistry - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)
    This data was developed using ab180125, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of acetone-fixed Jurkat cells labeling E1 Ubiquitin Activating Enzyme with ab180125 at 1/100 dilution, followed by Goat anti rabbit IgG (Dylight 488) at 1/250 dilution. Counter stained with DAPI (blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)
    This data was developed using ab180125, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling E1 Ubiquitin Activating Enzyme with ab180125 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)
    Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)

© 2021 Astana Biomed Group LLP. All rights reserved.