Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)
![Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)](https://www.abcam.com/ps/products/250/ab250138/Images/ab250138-408326-anti-e1-ubiquitin-activating-enzyme-1-uba1-antibody-epr14203-b-bsa-and-azide-free-western-blot.jpg "Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14203(B)] to E1 Ubiquitin Activating Enzyme 1/UBA1 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), IHC-P, ICC, WB
- Reacts with: Human
Overview
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Product name
Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free
See all E1 Ubiquitin Activating Enzyme 1/UBA1 primary antibodies -
Description
Rabbit monoclonal [EPR14203(B)] to E1 Ubiquitin Activating Enzyme 1/UBA1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, ICC, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250138 is the carrier-free version of ab180125.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14203(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)All lanes : Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] (ab180125) at 1/5000 dilution
Lane 1 : Fetal brain tissue lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 118 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?This data was developed using ab180125, the same antibody clone in a different buffer formulation.
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![Flow Cytometry - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)](https://www.abcam.com/ps/products/250/ab250138/Images/ab250138-3-anti-e1-ubiquitin-activating-enzyme-1-uba1-antibody-epr14203-b-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)This data was developed using ab180125, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling E1 Ubiquitin Activating Enzyme with ab180125 at 1/70 dilution (red) compared to a rabbit IgG control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/75 dilution. -
![Immunocytochemistry - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)](https://www.abcam.com/ps/products/250/ab250138/Images/ab250138-2-anti-e1-ubiquitin-activating-enzyme-1-uba1-antibody-epr14203-b-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)This data was developed using ab180125, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of acetone-fixed Jurkat cells labeling E1 Ubiquitin Activating Enzyme with ab180125 at 1/100 dilution, followed by Goat anti rabbit IgG (Dylight 488) at 1/250 dilution. Counter stained with DAPI (blue). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)](https://www.abcam.com/ps/products/250/ab250138/Images/ab250138-1-anti-e1-ubiquitin-activating-enzyme-1-uba1-antibody-epr14203-b-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)This data was developed using ab180125, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling E1 Ubiquitin Activating Enzyme with ab180125 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
![Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)](https://www.abcam.com/ps/products/250/ab250138/Images/ab250138-5-benefits-of-recombinant-antibodies.png)
Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14203(B)] - BSA and Azide free (ab250138)
