Anti-cleaved C-terminal GSDMD antibody [EPR20885-203] - BSA and Azide free (ab255984)
![Anti-cleaved C-terminal GSDMD antibody [EPR20885-203] - BSA and Azide free (ab255984)](https://www.abcam.com/ps/products/255/ab255984/Images/ab255984-349782-anticleavedcterminalgsdmdantibodyepr20885203westernblotsihahuman.jpg "Anti-cleaved C-terminal GSDMD antibody [EPR20885-203] - BSA and Azide free (ab255984)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20885-203] to cleaved C-terminal GSDMD - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-cleaved C-terminal GSDMD antibody [EPR20885-203] - BSA and Azide free
See all cleaved C-terminal GSDMD primary antibodies -
Description
Rabbit monoclonal [EPR20885-203] to cleaved C-terminal GSDMD - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SiHa (treated with 5 µg LPS/10E6 cells by electroporation for 2h.), cell lysate plus concentrated supernatant.
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General notes
Ab255984 is the carrier-free version of ab227821. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab255984 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20885-203 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-cleaved C-terminal GSDMD antibody [EPR20885-203] - BSA and Azide free (ab255984)All lanes : Anti-cleaved C-terminal GSDMD antibody [EPR20885-203] (ab227821) at 1/1000 dilution
Lane 1 : SiHa (human cervical squamous cell carcinoma), cell lysate plus concentrated cell supernatant
Lane 2 : SiHa (treated with 5 µg LPS/ 10E6 cells by electroporation for 2h.), cell lysate plus concentrated supernatant.
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution
Predicted band size: 53 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutesThe image is kindly provided by Dr Feng Shao.
The molecular weight observed is consistent with literature (PMID: 26375003).
Blocking/Dilution buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227821).
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![Anti-cleaved C-terminal GSDMD antibody [EPR20885-203] - BSA and Azide free (ab255984)](https://www.abcam.com/ps/products/255/ab255984/Images/ab255984-2-benefits-of-recombinant-antibodies.png)
Anti-cleaved C-terminal GSDMD antibody [EPR20885-203] - BSA and Azide free (ab255984)
