Anti-Grp75/MOT antibody [EPR5326(2)] - BSA and Azide free (ab248348)
![Anti-Grp75/MOT antibody [EPR5326(2)] - BSA and Azide free (ab248348)](https://www.abcam.com/ps/products/248/ab248348/Images/ab248348-402422-anti-grp75-mot-antibody-epr5326-2-bsa-and-azide-free-western-blot.jpg "Anti-Grp75/MOT antibody [EPR5326(2)] - BSA and Azide free (ab248348)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5326(2)] to Grp75/MOT - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Grp75/MOT antibody [EPR5326(2)] - BSA and Azide free
See all Grp75/MOT primary antibodies -
Description
Rabbit monoclonal [EPR5326(2)] to Grp75/MOT - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab248348 is the carrier-free version of ab129201. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab248348 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 4.30 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR5326(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Grp75/MOT antibody [EPR5326(2)] - BSA and Azide free (ab248348)All lanes : Anti-Grp75/MOT antibody [EPR5326(2)] (ab129201) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lane 4 : MCF-7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?This data was developed using ab129201, the same antibody clone in a different buffer formulation.
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![OI-RD Scanning - Anti-Grp75/MOT antibody [EPR5326(2)] - BSA and Azide free (ab248348)](https://www.abcam.com/ps/products/248/ab248348/Images/ab248348-1-anti-grp75-mot-antibody-epr5326-2-bsa-and-azide-free-equilibrium-disassociation-constant-kd.jpg)
OI-RD Scanning - Anti-Grp75/MOT antibody [EPR5326(2)] - BSA and Azide free (ab248348)This data was developed using ab129201, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
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![Anti-Grp75/MOT antibody [EPR5326(2)] - BSA and Azide free (ab248348)](https://www.abcam.com/ps/products/248/ab248348/Images/ab248348-3-benefits-of-recombinant-antibodies.png)
Anti-Grp75/MOT antibody [EPR5326(2)] - BSA and Azide free (ab248348)