Anti-BROX antibody (ab122174)
Key features and details
- Rabbit polyclonal to BROX
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BROX antibody -
Description
Rabbit polyclonal to BROX -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: LGNLVKNTLE KCQRENGFIY FQKIPTEAPQ LELKANYGLV EPIPFEFPPT SVQWTPETLA AFDLTKRPKD DSTKPKPEEE VKPVKEPDIK PQKDTGCYIS, corresponding to amino acids of Human BROX
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Positive control
- ICC/IF: U-2 OS cells. IHC-P: Human urinary bladder tissue. WB: RT4 and U-251 MG cell lysate. Human liver and tonsil tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-BROX antibody (ab122174)
Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli, cytoplasm and golgi apparatus. Recommended concentration of ab122174 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BROX antibody (ab122174)ab122174, at 1/500 dilution, staining BROX in paraffin-embedded human urinary bladder tissue by Immunohistochemistry.
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Western blot - Anti-BROX antibody (ab122174)All lanes : Anti-BROX antibody (ab122174) at 1/250 dilution
Lane 1 : RT 4 lysate
Lane 2 : U 251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.