Mouse P-Selectin ELISA Kit (CD62P) (ab200014)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 4.16 pg/ml
- Range: 23.4 pg/ml - 1500 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse P-Selectin ELISA Kit (CD62P)
See all CD62P kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 5.6% Inter-assay Sample n Mean SD CV% Serum 3 3.21% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
4.16 pg/ml -
Range
23.4 pg/ml - 1500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 112 107% - 116% Cell culture media 119 118% - 120% Hep Plasma 127 119% - 139% EDTA Plasma 122 119% - 126% Cit plasma 114 103% - 120% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Goat, Cow, Pig -
Product overview
Mouse P-Selectin ELISA Kit (CD62P) (ab200014) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of P-Selectin (CD62P) protein in cit plasma, serum, cell culture supernatant, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse P-Selectin (CD62P) with 4.16 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
P-Selectin is the largest member of the selectin family, which are adhesion molecules that ensure efficient recruitment of leukocytes to inflammation sites. It is stored in platelets and endothelial cells in Weibel-Palade bodies and α-granules, and is expressed by membrane translocation. P-Selectin’s main function is to mediate the interaction of activated endothelial cells or platelets with leukocytes, and most importantly, leukocyte rolling after trauma. High soluble levels of P-Selectin are thought to be predictive of future cardiovascular events.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse P-Selectin Capture Antibody 1 x 600µl 10X Mouse P-Selectin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CP 1 x 6ml Mouse P-Selectin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. -
Tissue specificity
Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface. -
Involvement in disease
Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. -
Sequence similarities
Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 9 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Antigen CD62
- CD62
- CD62 antigen-like family member P
see all -
Database links
- Entrez Gene: 20344 Mouse
- SwissProt: Q01102 Mouse
- Unigene: 3337 Mouse
Images
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Other - Mouse P-Selectin ELISA Kit (CD62P) (ab200014)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse P-Selectin (CD62P) standard curve comparison data.Standard curve comparison between mouse P-Selectin (CD62P) SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 4-fold increase in sensitivity.
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Example of P-Selectin standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of P-Selectin standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Titration of mouse serum, EDTA platelet poor plasma, citrate plasma, and heparin plasma within the working range of the assay.The 1X dilution for serum and each plasma was at 2% matrix. The interpolated P-selectin concentrations multiplied by the total dilution factor are shown for each matrix (mean +/- SD, n = 2).
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Titration of lung and spleen extracts in 1X Cell Extraction Buffer PTR within the working range of the assay.The 1X dilution for the lung extracts was 500 µg/mL, and for the spleen extracts 125 µg/mL. The interpolated P-Selectin concentrations multiplied by the total dilution factor are shown for each supernatant (mean +/- SD, n = 2).
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Sandwich ELISA - Mouse P-Selectin ELISA Kit (CD62P) (ab200014)To learn more about the advantages of recombinant antibodies see here.
