Anti-RBM33 antibody (ab121695)
Key features and details
- Rabbit polyclonal to RBM33
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RBM33 antibody
See all RBM33 primary antibodies -
Description
Rabbit polyclonal to RBM33 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
antigen sequence: QHPPQHQHHH HHHHLSVPPP PLMPMSQPQF RPHVQTAQPQ ASSSRMQCPQ RQGLRHNTTS QNVSKRPMQQ MQPTAPRNSN LREL, corresponding to amino acids 628-711 of Human RBM33
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Positive control
- Human rectum tissue; RT-4 cells lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-RBM33 antibody (ab121695)Immunofluorescent staining of Human cell line U-251MG shows positivity in nuclei but not nucleoli. Recommended concentration of ab121695 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Western blot - Anti-RBM33 antibody (ab121695)All lanes : Anti-RBM33 antibody (ab121695) at 1/100 dilution
Lane 1 : NIH-3T3 cell lysate (Mouse embryonic cells)
Lane 2 : NBT-II cell lysate (Rat Wistar bladder tumour cells)
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Western blot - Anti-RBM33 antibody (ab121695)All lanes : Anti-RBM33 antibody (ab121695) at 1/250 dilution
Lane 1 : Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2 : RT-4 cells lysate
Lane 3 : U-251 MG cells lysate
Lane 4 : Human plasma lysate
Lane 5 : Liver lysate
Lane 6 : Tonsil lysate
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM33 antibody (ab121695)Immunohistochemical staining of paraffin embedded Human rectum tissue, using 1/40 ab121695.
