Anti-C1orf93 antibody [EPR13703] (ab180932)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13703] to C1orf93
- Suitable for: IP, WB
- Reacts with: Human
Overview
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Product name
Anti-C1orf93 antibody [EPR13703]
See all C1orf93 primary antibodies -
Description
Rabbit monoclonal [EPR13703] to C1orf93 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Fetal kidney, HeLa, MCF7 and THP1 cell lines.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13703 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-C1orf93 antibody [EPR13703] (ab180932) at 1/5000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 21 kDa
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Anti-C1orf93 antibody [EPR13703] (ab180932) at 1/1000 dilution + THP1 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 21 kDa
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Immunoprecipitation of fetal kidney cell line labeling C1orf93 using ab180932 at a 1/60 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as a secondary antibody at a dilution of 1/1500. Blocking/dilution buffer and concentration: 5% NFDM/TBST
All lanes : Anti-C1orf93 antibody [EPR13703] (ab180932) at 1/60 dilution
Lane 1 : fetal kidney cell line at 10 µg
Lane 2 : Negative control
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution
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