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Immunology Adaptive Immunity T Cells Cytotoxic Cells

Anti-SET/TAF-I antibody (ab92872)

Price and availability

301 536 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-SET/TAF-I antibody (ab92872)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to SET/TAF-I
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SET/TAF-I antibody
    See all SET/TAF-I primary antibodies
  • Description

    Rabbit polyclonal to SET/TAF-I
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to Human SET/TAF-I aa 125-175.
    Sequence:

    VTEFEDIKSG YRIDFYFDEN PYFENKVLSK EFHLNESGDP SSKSTEIKWK S


    Database link: NP_001116293.1
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HeLa and 293T cell lysate
  • General notes

     This product was previously labelled as SET

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Cytotoxic Cells
    • Signal Transduction
    • Protein Trafficking
    • ER Proteins
    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Histone chaperones
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Other cell division antibodies
    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Chromatin assembly
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Other

Images

  • Western blot - Anti-SET/TAF-I antibody (ab92872)
    Western blot - Anti-SET/TAF-I antibody (ab92872)
    All lanes : Anti-SET/TAF-I antibody (ab92872) at 0.04 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 33 kDa


    Exposure time: 30 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody (ab92872)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody (ab92872)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SET/TAF-I with ab92872 at 1/400 (0.5µg/ml). A DyLight® 594-conjugated goat anti-rabbit IgG (H+L) (1/100) was used as the secondary antibody.

  • Immunoprecipitation - Anti-SET/TAF-I antibody (ab92872)
    Immunoprecipitation - Anti-SET/TAF-I antibody (ab92872)

    3µg ab92872 were used to immunoprecipitate SET/TAF-I from 1mg HeLa whole cell lysate. 20% IP was loaded and probed with ab92872 at 1µg/ml.

  • Western blot - Anti-SET/TAF-I antibody (ab92872)
    Western blot - Anti-SET/TAF-I antibody (ab92872) This image is courtesy of an anonymous Abreview
    Anti-SET/TAF-I antibody (ab92872) at 1/2000 dilution ((in 3% milk in PBST). Incubation was for 24 hours at 4°C.) + Lysate of the soluble fraction of HeLa or U20S cells at 30 µg

    Secondary
    An HRP polymer Goat anti-rabbit IgG polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 33 kDa
    Observed band size: ~37,~40 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking Step: 5% Milk for 30 minutes at room temperature.

    ab92872 recognises both isoforms of human SET/TAF-I.

    See Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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