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Immunology Adaptive Immunity T Cells Cytotoxic Cells

Anti-SET/TAF-I antibody [EPR12972(B)] (ab176567)

Price and availability

311 587 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-SET/TAF-I antibody [EPR12972(B)] (ab176567)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12972(B)] to SET/TAF-I
  • Suitable for: IP, Flow Cyt, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-SET/TAF-I antibody [EPR12972(B)]
    See all SET/TAF-I primary antibodies
  • Description

    Rabbit monoclonal [EPR12972(B)] to SET/TAF-I
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human SET/TAF-I aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q01105

  • Positive control

    • Jurkat, 293T, HepG2 and HeLa whole cell lysate (ab150035); Jurkat cells.
  • General notes

     This product was previously labelled as SET

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12972(B)
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Cytotoxic Cells
    • Signal Transduction
    • Protein Trafficking
    • ER Proteins
    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Histone chaperones
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Other cell division antibodies
    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Chromatin assembly
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Other

Images

  • Western blot - Anti-SET/TAF-I antibody [EPR12972(B)] (ab176567)
    Western blot - Anti-SET/TAF-I antibody [EPR12972(B)] (ab176567)
    All lanes : Anti-SET/TAF-I antibody [EPR12972(B)] (ab176567) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 33 kDa

  • Flow Cytometry - Anti-SET/TAF-I antibody [EPR12972(B)] (ab176567)
    Flow Cytometry - Anti-SET/TAF-I antibody [EPR12972(B)] (ab176567)

    Flow cytometry analysis of permeabilized Jurkat cells labeling SET/TAF-I (red) with ab176567 at a 1/10 dilution, or negative control rabbit IgG (green)

  • Immunoprecipitation - Anti-SET/TAF-I antibody [EPR12972(B)] (ab176567)
    Immunoprecipitation - Anti-SET/TAF-I antibody [EPR12972(B)] (ab176567)

    Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling SET/TAF-I with ab176567 at 1/10 dilution.

  • Anti-SET/TAF-I antibody [EPR12972(B)] (ab176567)
    Anti-SET/TAF-I antibody [EPR12972(B)] (ab176567)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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