Antibodies, Proteins, Kits and Reagents for Life Science

Anti-SET/TAF-I antibody [EPR12973] - BSA and Azide free (ab235387)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12973] to SET/TAF-I - BSA and Azide free
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
Reacts with: Rat, Human

Product name

Anti-SET/TAF-I antibody [EPR12973] - BSA and Azide free
See all SET/TAF-I primary antibodies
Description

Rabbit monoclonal [EPR12973] to SET/TAF-I - BSA and Azide free
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Rat
IP	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
ab235387 is the carrier-free version of ab181990 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab235387 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

This product was previously labelled as SET
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12973
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-SET/TAF-I antibody [EPR12973] - BSA and Azide free (ab235387)

Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling SET/TAF-I with Purified ab181990 at 1/500 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181990).
Immunoprecipitation - Anti-SET/TAF-I antibody [EPR12973] - BSA and Azide free (ab235387)

Immunoprecipitation of Jurkat cell lysate labeling SET/TAF-I using ab181990 at a 1/30 dilution (lane 1). A goat anti-rabbit IgG, (H+L), peroxidase conjugated secondary antibody was used at a dilution of 1/1000. Blocking/dilution buffer and concentration: 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181990).
Flow Cytometry - Anti-SET/TAF-I antibody [EPR12973] - BSA and Azide free (ab235387)

Flow cytometric analysis of 2% paraformaldehyde-fixed Hela cells labeling SET/TAF-I using ab181990 at a 1/10 dilution, or a rabbit monoclonal IgG isotype control. Secondary antibody used was goat anti rabbit IgG (FITC) at a 1/150 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181990).
Immunocytochemistry/ Immunofluorescence - Anti-SET/TAF-I antibody [EPR12973] - BSA and Azide free (ab235387)

Immunofluorescent analysis of 4% paraformaldehyde fixed HepG2 cell line staining SET/TAF-I using ab181990 at 1/1000 dilution. A Dylight 555 conjugated Goat anti rabbit IgG at 1/200 dilution was used for secondary antibody detection. Counter stain: Dapi.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181990).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody [EPR12973] - BSA and Azide free (ab235387)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling SET/TAF-I with ab181990 at a 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181990).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-SET/TAF-I antibody [EPR12973] - BSA and Azide free (ab235387)

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