Anti-Annexin V/ANXA5 antibody (ab54775)
Key features and details
- Mouse monoclonal to Annexin V/ANXA5
- Suitable for: IP, WB, ICC/IF, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Annexin V/ANXA5 antibody
See all Annexin V/ANXA5 primary antibodies -
Description
Mouse monoclonal to Annexin V/ANXA5 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human Annexin V/ANXA5 aa 1-321.
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General notes
This product was changed from ascites to tissue culture supernatant on 22/03/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
This product was previously labelled as Annexin V.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Annexin V/ANXA5 antibody (ab54775)
ab54775 Western Blot analysis of ANXA5/ANXA5 expression in Hela cells.
This image was generated using the ascites version of the product.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin V/ANXA5 antibody (ab54775)Annexin V/ANXA5 antibody (ab54775) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon.
This image was generated using the ascites version of the product.
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Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody (ab54775)ab54775 at 10 ug/ml staining Annexin V/ANXA5 in human Hela cells by Immunocytochemistry / Immunofluorescence.
This image was generated using the ascites version of the product.
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Flow Cytometry - Anti-Annexin V/ANXA5 antibody (ab54775)Overlay histogram showing HeLa cells stained with ab54775 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54775, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
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Immunoprecipitation - Anti-Annexin V/ANXA5 antibody (ab54775)Immunoprecipitation of ANXA5 transfected lysate using ab54775 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with a ANXA5 rabbit polyclonal antibody.
This image was generated using the ascites version of the product.
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Western blot - Anti-Annexin V/ANXA5 antibody (ab54775)Western Blot analysis of ANXA5 expression in transfected 293T cell line by ab54775.
Lane 1: ANXA5 transfected lysate(35.9 KDa).
Lane 2: Non-transfected lysate.This image was generated using the ascites version of the product.
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Immunocytochemistry/ Immunofluorescence - Anti-Annexin V/ANXA5 antibody (ab54775)ab54775 staining Annexin V/ANXA5 in HeLa cells treated with pentoxifylline (ab120725), by ICC/IF. Increase of Annexin V/ANXA5 correlates with increased concentration of pentoxifylline, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120725 (pentoxifylline) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab54775 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.This image was generated using the ascites version of the product.
