Anti-Annexin-2/ANXA2 antibody [D1/274.5] (ab269511)
![Anti-Annexin-2/ANXA2 antibody [D1/274.5] (ab269511)](https://www.abcam.com/ps/products/269/ab269511/Images/ab269511-391691-anti-annexin-ii-antibody-d12745-western-blot-human-ovary-cancer-tissue-lysate20-ug.jpg "Anti-Annexin-2/ANXA2 antibody [D1/274.5] (ab269511)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [D1/274.5] to Annexin-2/ANXA2
- Suitable for: ICC/IF, WB
- Reacts with: Human
Overview
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Product name
Anti-Annexin-2/ANXA2 antibody [D1/274.5]
See all Annexin-2/ANXA2 primary antibodies -
Description
Mouse monoclonal [D1/274.5] to Annexin-2/ANXA2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Tissue, cells or virus within Human Annexin-2/ANXA2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- WB: Human ovary cancer tissue lysate. HeLa and HUVEC whole cell lysate. ICC/IF: HeLa and MCF7 cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
D1/274.5 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-Annexin-2/ANXA2 antibody [D1/274.5] (ab269511)All lanes : Anti-Annexin-2/ANXA2 antibody [D1/274.5] (ab269511) at 0.773 µg/ml
Lane 1 : Human ovary cancer tissue lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : HUVEC (human umbilical vein endothelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 38 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Lane 2-3 of this blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 minutes.
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![Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [D1/274.5] (ab269511)](https://www.abcam.com/ps/products/269/ab269511/Images/ab269511-391678-antiannexiniiantibodyd12745immunocytochemistrymcf7.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [D1/274.5] (ab269511)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells labelling Annexin II with ab269511 at 15.46 µg/mL. Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution., followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strongly membranous and cytoplasmic staining in MCF7 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
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![Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [D1/274.5] (ab269511)](https://www.abcam.com/ps/products/269/ab269511/Images/ab269511-1-anti-annexin-ii-antibody-d12745-immunocytochemistry-hela.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Annexin-2/ANXA2 antibody [D1/274.5] (ab269511)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Annexin II with ab269511 at 15.46 µg/mL, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strongly membranous and cytoplasmic staining in HeLa cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Anti-Annexin-2/ANXA2 antibody [D1/274.5] (ab269511)](https://www.abcam.com/ps/products/269/ab269511/Images/ab269511-4-benefits-of-recombinant-antibodies.png)
Anti-Annexin-2/ANXA2 antibody [D1/274.5] (ab269511)