Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Annexin A10+Annexin A11 antibody [EPR19442] - BSA and Azide free (ab251580)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19442] to Annexin A10+Annexin A11 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Annexin A10+Annexin A11 antibody [EPR19442] - BSA and Azide free
    See all Annexin A10+Annexin A11 primary antibodies

  • Description

    Rabbit monoclonal [EPR19442] to Annexin A10+Annexin A11 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251580 is the carrier-free version of ab223848. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251580 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10+Annexin A11 antibody [EPR19442] - BSA and Azide free (ab251580)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10+Annexin A11 antibody [EPR19442] - BSA and Azide free (ab251580)

    This data was developed using ab223848, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Annexin A10 + Annexin A11 with ab223848 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear and cytoplasmic staining in human stomach (PMID: 24024557; PMID: 21175800). Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
  • Western blot - Anti-Annexin A10+Annexin A11 antibody [EPR19442] - BSA and Azide free (ab251580)
    Western blot - Anti-Annexin A10+Annexin A11 antibody [EPR19442] - BSA and Azide free (ab251580)
    All lanes : Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848) at 1/1000 dilution

    Lane 1 : HT-1376 (human urinary bladder carcinoma cell line) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 5 : Human stomach lysate
    Lane 6 : Human pancreas lysate
    Lane 7 : BxPC-3 (human pancreas adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 5-7 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 37,54 kDa
    Observed band size: 35,54 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab223848, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-4, 6, and 7: 3 minutes; Lane 5: 30 seconds.

    The molecular mass observed (35kDa) is consistent with what has been described in the literature (PMID: 21175800).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10+Annexin A11 antibody [EPR19442] - BSA and Azide free (ab251580)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10+Annexin A11 antibody [EPR19442] - BSA and Azide free (ab251580)
    This data was developed using ab223848, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling Annexin A10 + Annexin A11 with ab223848 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear and weak cytoplasmic staining in human gastric cancer (PMID:24024557; PMID: 21175800). Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
  • Western blot - Anti-Annexin A10+Annexin A11 antibody [EPR19442] - BSA and Azide free (ab251580)
    Western blot - Anti-Annexin A10+Annexin A11 antibody [EPR19442] - BSA and Azide free (ab251580)
    All lanes : Anti-Annexin A10/ANXA10 + Annexin-11/ANXA11 antibody [EPR19442] (ab223848) at 1/10000 dilution

    Lane 1 : His-tagged human Annexin A10 recombinant protein (aa134-324)
    Lane 2 : His-tagged human Annexin A11 recombinant protein (aa1-505)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 37,54 kDa
    Observed band size: 20,54 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This data was developed using ab223848, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    This product cross-reacts with Annexin A11.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10+Annexin A11 antibody [EPR19442] - BSA and Azide free (ab251580)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10+Annexin A11 antibody [EPR19442] - BSA and Azide free (ab251580)
    This data was developed using ab223848, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Annexin A10 + Annexin A11 with ab223848 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear and weak cytoplasmic staining in human bladder cancer (PMID: 24024557). Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10+Annexin A11 antibody [EPR19442] - BSA and Azide free (ab251580)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A10+Annexin A11 antibody [EPR19442] - BSA and Azide free (ab251580)
    This data was developed using ab223848, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Annexin A10 + Annexin A11 with ab223848 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear and cytoplasmic staining in rat stomach. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
  • Anti-Annexin A10+Annexin A11 antibody [EPR19442] - BSA and Azide free (ab251580)
    Anti-Annexin A10+Annexin A11 antibody [EPR19442] - BSA and Azide free (ab251580)

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