Anti-Annexin A1/ANXA1 antibody (ab137745)
Key features and details
- Rabbit polyclonal to Annexin A1/ANXA1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Annexin A1/ANXA1 antibody
See all Annexin A1/ANXA1 primary antibodies -
Description
Rabbit polyclonal to Annexin A1/ANXA1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow, Pig -
Immunogen
Recombinant fragment corresponding to Human Annexin A1/ANXA1 aa 24-346 (internal sequence).
Database link: P04083 -
Positive control
- A431, H1299, HeLa, MOLT4, NIH-3T3 and JC whole cell lysates; HeLa cells; N87 xenograft tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Annexin A1/ANXA1 antibody (ab137745) at 1/30000 dilution
Lane 1 : Wild-type HeLa whole cell lysate
Lane 2 : Annexin A1/ANXA1 knockout HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 39 kDa10% SDS-PAGE
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All lanes : Anti-Annexin A1/ANXA1 antibody (ab137745) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : H1299 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
10% SDS PAGE -
All lanes : Anti-Annexin A1/ANXA1 antibody (ab137745) at 1/10000 dilution
Lane 1 : NIH-3T3 cell lysate
Lane 2 : JC cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
10% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labelling Annexin A1 with ab137745 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
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Immunohistochemical analysis of paraffin-embedded N87 Xenograft, labelling Annexin A1 using ab137745 at 1/500 dilution.