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Signal Transduction Cytoskeleton / ECM Cell Adhesion Annexins

Anti-Annexin A1/ANXA1 antibody (ab137745)

Price and availability

284 784 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-Annexin A1/ANXA1 antibody (ab137745)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Annexin A1/ANXA1
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Annexin A1/ANXA1 antibody
    See all Annexin A1/ANXA1 primary antibodies
  • Description

    Rabbit polyclonal to Annexin A1/ANXA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Cow, Pig
  • Immunogen

    Recombinant fragment corresponding to Human Annexin A1/ANXA1 aa 24-346 (internal sequence).
    Database link: P04083

  • Positive control

    • A431, H1299, HeLa, MOLT4, NIH-3T3 and JC whole cell lysates; HeLa cells; N87 xenograft tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Annexins
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Other
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Other

Images

  • Western blot - Anti-Annexin A1/ANXA1 antibody (ab137745)
    Western blot - Anti-Annexin A1/ANXA1 antibody (ab137745)
    All lanes : Anti-Annexin A1/ANXA1 antibody (ab137745) at 1/30000 dilution

    Lane 1 : Wild-type HeLa whole cell lysate
    Lane 2 : Annexin A1/ANXA1 knockout HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 39 kDa



    10% SDS-PAGE

  • Western blot - Anti-Annexin A1/ANXA1 antibody (ab137745)
    Western blot - Anti-Annexin A1/ANXA1 antibody (ab137745)
    All lanes : Anti-Annexin A1/ANXA1 antibody (ab137745) at 1/1000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : H1299 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 39 kDa



    10% SDS PAGE
  • Western blot - Anti-Annexin A1/ANXA1 antibody (ab137745)
    Western blot - Anti-Annexin A1/ANXA1 antibody (ab137745)
    All lanes : Anti-Annexin A1/ANXA1 antibody (ab137745) at 1/10000 dilution

    Lane 1 : NIH-3T3 cell lysate
    Lane 2 : JC cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 39 kDa



    10% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-Annexin A1/ANXA1 antibody (ab137745)
    Immunocytochemistry/ Immunofluorescence - Anti-Annexin A1/ANXA1 antibody (ab137745)
    Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labelling Annexin A1 with ab137745 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody (ab137745)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Annexin A1/ANXA1 antibody (ab137745)
    Immunohistochemical analysis of paraffin-embedded N87 Xenograft, labelling Annexin A1 using ab137745 at 1/500 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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