ANXA7 overexpression 293T lysate (whole cell) (ab94175)
Overview
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Product name
ANXA7 overexpression 293T lysate (whole cell) -
General notes
ab94175 is a 293T cell transfected lysate in which Human Annexin VII has been transiently over-expressed using a pCMV-Annexin VII plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. Tissue specificity: Isoform 1 is expressed in brain, heart and skeletal muscle. Isoform 2 is more abundant in liver, lung, kidney, spleen, fibroblasts and placenta. Similarity: Belongs to the annexin family. Contains 4 annexin repeats. Domain: A pair of annexin repeats may form one binding site for calcium and phospholipid.
Images
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SDS-PAGE - ANXA7 overexpression 293T lysate (whole cell) (ab94175)ab94175 at 15µg/lane on an SDS-PAGE gel.
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Western blot - ANXA7 overexpression 293T lysate (whole cell) (ab94175)All lanes : Anti-Annexin-7/ANXA7 antibody (ab88539) at 1/500 dilution
Lane 1 :ANXA7 overexpression 293T lysate (whole cell) (ab94175)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
