Anti-S100A4 antibody [ABM48F7] (ab228445)
![Anti-S100A4 antibody [ABM48F7] (ab228445)](https://www.abcam.com/ps/products/228/ab228445/Images/ab228445-353590-antis100a4antibodyepr27612westernblotknockouthelas100a4a375a549humanlysates228445gdb.jpg "Anti-S100A4 antibody [ABM48F7] (ab228445)")
Key features and details
- Mouse monoclonal [ABM48F7] to S100A4
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-S100A4 antibody [ABM48F7]
See all S100A4 primary antibodies -
Description
Mouse monoclonal [ABM48F7] to S100A4 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant full length protein corresponding to Human S100A4 aa 1 to the C-terminus.
Database link: P26447 -
Positive control
- WB: A549 cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
ABM48F7 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-S100A4 antibody [ABM48F7] (ab228445)All lanes : Anti-S100A4 antibody [ABM48F7] (ab228445) at 1 µg/ml
Lane 1 : Wild-type A549 whole cell lysate
Lane 2 : S100A4 knockout A549 whole cell lysate
Lane 3 : A375 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 12 kDa
Observed band size: 12 kDaLanes 1 - 4: Merged signal (red and green). Green - ab228445 observed at 12 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab228445 was shown to recognize S100A4 in wild-type A549 cells as signal was lost at the expected MW in S100A4 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and S100A4 knockout samples were subjected to SDS-PAGE. Ab228445 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-S100A4 antibody [ABM48F7] (ab228445)](https://www.abcam.com/ps/products/228/ab228445/Images/ab228445-293070-ab2284451.jpg)
Western blot - Anti-S100A4 antibody [ABM48F7] (ab228445)Anti-S100A4 antibody [ABM48F7] (ab228445) at 0.5 µg/ml + A549 (human lung carcinoma cell line) cell lysate
Predicted band size: 12 kDa
