Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Bag3 antibody [EPR3515] (ab92309)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3515] to Bag3
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Bag3 antibody [EPR3515]
    See all Bag3 primary antibodies

  • Description

    Rabbit monoclonal [EPR3515] to Bag3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Bag3 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • 293T, HeLa, MCF7 and K562 cell lysates. IP: Mouse heart lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Bag3 antibody [EPR3515] (ab92309)
    Western blot - Anti-Bag3 antibody [EPR3515] (ab92309)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: BAG3 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: K562 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab92309 observed at 80 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab92309 was shown to recognize BAG3 in wild-type cells as signal was lost at the expected MW in BAG3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and BAG3 knockout samples were subjected to SDS-PAGE. Ab92309 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-Bag3 antibody [EPR3515] (ab92309)
    Immunoprecipitation - Anti-Bag3 antibody [EPR3515] (ab92309)

    Purified ab92309 at 1/50 dilution (2µg) immunoprecipitating Bag3 in Mouse heart lysate.
    Lane 1 (input): Mouse heart lysate 10µg
    Lane 2 (+): ab92309 + Mouse heart lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92309 in Mouse heart lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 80 kDa

  • Western blot - Anti-Bag3 antibody [EPR3515] (ab92309)
    Western blot - Anti-Bag3 antibody [EPR3515] (ab92309)
    All lanes : Anti-Bag3 antibody [EPR3515] (ab92309) at 1/1000 dilution

    Lane 1 : 293T cell
    lysate
    Lane 2 : HeLa cell
    lysate
    Lane 3 :
    MCF7 cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 62 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?

  • Anti-Bag3 antibody [EPR3515] (ab92309)
    Anti-Bag3 antibody [EPR3515] (ab92309)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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