Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PD-L1 antibody [CAL10] - Chimeric – BSA and Azide free (ab279305)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [CAL10] to PD-L1 - Chimeric – BSA and Azide free
  • Suitable for: WB, Flow Cyt (Intra), ICC, IHC-P
  • Reacts with: Human, Chinese hamster

Overview

  • Product name

    Anti-PD-L1 antibody [CAL10] - Chimeric – BSA and Azide free
    See all PD-L1 primary antibodies

  • Description

    Mouse monoclonal [CAL10] to PD-L1 - Chimeric – BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, Flow Cyt (Intra), ICC, IHC-Pmore details

  • Species reactivity

    Reacts with: Human, Chinese hamster

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: PD-L1 stably expressed CHO whole cell lysate. Human placenta tissue lysate. NCI-H1299 whole cell lysate. ICC: PD-L1 stably expressed CHO cells. Flow Cyt: PD-L1 stably expressed CHO cells. IHC-P: Human tonsil tissue.

  • General notes

    ab279305 is the carrier free version of ab279293.

    This mouse antibody has been engineered from a RabMAb parent antibody (ab237726). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-PD-L1 antibody [CAL10] - Chimeric – BSA and Azide free (ab279305)
    Western blot - Anti-PD-L1 antibody [CAL10] - Chimeric – BSA and Azide free (ab279305)
    All lanes : Anti-PD-L1 antibody [CAL10] (ab279293) at 1/1000 dilution

    Lane 1 : CHO-S (Chinese hamster ovary epithelial cell) whole cell lysate
    Lane 2 : CHO-PD-L1 (PD-L1 stably expressed Chinese hamster ovary epithelial cell) whole cell lysate
    Lane 3 : Human placenta tissue lysate
    Lane 4 : NCI-H1299 (human lung carcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Observed band size: 40-60 kDa
    why is the actual band size different from the predicted?



    This data was produced using ab279293, the same clone in a different formulation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [CAL10] - Chimeric – BSA and Azide free (ab279305)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [CAL10] - Chimeric – BSA and Azide free (ab279305)

    This data was produced using ab279293, the same clone in a different formulation.

    IHC image of PD-L1 staining in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BONDTM system using the standard protocol F.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab279293, 1ug/ml, for 15 mins at room temperature. A rabbit anti-mouse IgG2a, was added for 8 mins at room temperature and detected using an HRP conjugated goat anti-rabbit compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunocytochemistry - Anti-PD-L1 antibody [CAL10] - Chimeric – BSA and Azide free (ab279305)
    Immunocytochemistry - Anti-PD-L1 antibody [CAL10] - Chimeric – BSA and Azide free (ab279305)

    This data was produced using ab279293, the same clone in a different formulation.

    Immunocytochemical analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100-fixed permeabilized CHO-PD-L1 cells labeling PD-L1 with ab279293 at 1/100 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue). Confocal image showing membranous and cytoplasmic staining in CHO-PD-L1 cells.

    Negative control 1: ab279293 at a 1/100 dilution followed by ab150080 at a 1/200 dilution.

    Negative control 2: ab179513 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-PD-L1 antibody [CAL10] - Chimeric – BSA and Azide free (ab279305)
    Flow Cytometry (Intracellular) - Anti-PD-L1 antibody [CAL10] - Chimeric – BSA and Azide free (ab279305)

    This data was produced using ab279293, the same clone in a different formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized CHO-s (Chinese hamster ovary epithelial cell, Blue) / CHO-PDL1 (PD-L1 stably expessed Chinese hamster ovary epithelial cell, Red) labelling PD-L1 with ab279293 at 1/50 dilution (0.1µg).

    Goat Anti-Mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

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