Human Histone H3 (tri methyl K4) peptide (ab1342)
Key features and details
- Suitable for: Dot blot, Blocking
-
Product name
Human Histone H3 (tri methyl K4) peptide
See all Histone H3 proteins and peptides -
Accession
-
Animal free
No -
Nature
Synthetic -
-
Species
Human -
Modifications
tri methyl K4
-
Preparation and Storage
-
Applications
-
Form
Liquid -
Additional notes
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use. -
Concentration information loading...
Images
-
Dot Blot - Human Histone H3 (tri methyl K4) peptide (ab1342) This image is courtesy of an anonymous Abreview
ab1342 (at 0.01 and 0.1 µg) used in Dot Blot to test for cross-reactivity of ab8580 (1/2500). An HRP-conjugated Mouse anti-rabbit polyclonal (1/10000) was used as the secondary antibody.
